Nucleic Acids

Cy5.5 is a near-infrared fluorescent dye for labeling biomolecules such as peptides, proteins and oligonucleotides (Ex=673 nm, Em=707 nm)[2].

Propidium Iodide (PI) is a red fluorescent dye utilized for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of PI is 493/636 nm. Upon binding with nucleic acid, the Ex/Em shifts to 535/617 nm, enhancing the fluorescence signal 20-30 times.

Acridine Orange hydrochloride is a fluorescent dye of cell-permeable. It binds to nucleic acids, resulting in an altered spectral emission.It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.

BHQ3 Maleimide is a fluorescent probe that eliminates background fluorescence and can be used for protein labeling, nucleic acid labeling and cellular imaging.

Sulfo-Cy5.5 is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) that can be used to label biomolecules such as peptides, proteins, and oligonucleotides [2].

DFHO is a Corn homologous ligand that fluoresces yellow upon excitation and is used to image RNA in living cells.

CY3 (Sulfo-Cyanine3) is an orange-fluorescent label for nucleic acids and proteins (λex=554, λem=568).

1,8-Diazafluoren-9-one (DFO) is utilized for detecting fingerprints on porous surfaces.

6-ROX (6-Carboxy-X-rhodamine) is a fluorescent oligonucleotide marker and acts as an acceptor molecule coupled to 5-FAM as the donor in FRET imaging with excitation of 514nm and emission of 525nm.

Acridine Orange base is a cell-permeable fluorescent dye that binds to nucleic acids and emits different colors of fluorescence, which exhibits green fluorescence when bound to dsDNA (Ex=488 nm, Em=520-524 nm) and red fluorescence when bound to ssDNA or ssRNA (Ex=457 nm, Em=630-644 nm) and can be used for cell cycle analysis and apoptosis detection.

Pyronin Y (Pyronine G) is an intercalating cationic dye with specificity for RNA.

5-TRITC is an amine-reactive fluorescent dye commonly used to label proteins and nucleic acids. It reacts with amino groups in proteins to form stable thioether bonds, thereby labeling and detecting the presence and localization of specific proteins, producing bright red-orange fluorescence with excitation/emission maxima of about 555/580 nm.

Proflavine Hemisulfate (3,6-Diaminoacridine hemisulfate) is the hemisulfate salt form of proflavine, an acridine-derived fluorescent contrast and disinfectant agent that can potentially be used for cellular imaging and antiseptic purposes.

CY3-YNE (Sulfo-Cyanine3-alkyne) is a dye labeled with a biomarker group alkyne, which can be used to label proteins, antibodies, peptides, nucleic acid molecules, and nanoparticles (Ex/Em: 554/566 nm).

Dabcyl acid (DABCYL) is the original dark fluorescence quencher.

1-Methyl-7-nitroisatoic anhydride (1M7) is a reagent. It detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.

5-FAM SE is a single isomer widely employed in the preparation of various small fluorescent molecules. It is a highly popular green fluorescent reagent used for labeling peptides, proteins, and nucleotides [5-FAM SE].

1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission.

CelRed nucleic acid gel stain 10,000× concentrate in water is a fluorescent dye used for nucleic acid staining, suitable for staining and detecting dsDNA, ssDNA, or RNA in agarose gels or polyacrylamide gels.

Cyanine3 NHS Ester Tetrafluoroborate is a fluorescent labeling reagent containing Cy3 fluorophore, NHS ester active group and tetrafluoroborate stabilizer. Cyanine3 NHS Ester Tetrafluoroborate can react with amino groups on biological molecules and is used in biological analysis fields such as protein, antibody, nucleic acid labeling and cell imaging.

Cy5.5 diacid, SE is commonly used in biomolecular labeling, fluorescence imaging and other fluorescence biological analysis. Cy5.5 dyes and their derivatives are widely used to label polypeptides, proteins and nucleic acids.

Pyren-1-ylmethanamine hydrochloride is commonly used as a fluorescent probe in biochemistry and molecular biology to detect and study nucleic acids, proteins, and other biomolecules. It has also been used in analytical chemistry for the detection of heavy metals and other pollutants in environmental samples. The pyrene group in the molecule exhibits strong fluorescence properties, making it a sensitive and selective detector in various applications.

Fast Green free acid is a dye that resists acid once applied.

2-Aza-ε-adenosine (2-Aza-1,N6-ethenoadenosine) serves as a versatile dye with broad applications in various fields. As essential components in biological experimentation, dyes enable scientists to examine and track cell structures, monitor biomolecules, assess cellular functions, differentiate among cell types, explore tissue pathology, and scrutinize microorganisms. Their utility extends beyond biological research into clinical diagnostics, influencing a wide spectrum of industries including traditional textile dyeing, innovative textile processing, food coloring, and dye-sensitized solar cells.