BHQ3 Maleimide is a fluorescent probe that eliminates background fluorescence and can be used for protein labeling, nucleic acid labeling and cellular imaging.

Instructions
1. Protein labeling
Experimental steps:
1. Prepare a protein solution containing thiol groups to ensure that the protein concentration is suitable for the reaction.
2. Prepare the BHQ3 Maleimide solution, usually dissolved in an appropriate solvent (such as DMSO).
3. Add BHQ3 Maleimide solution to the protein solution, and the reaction time is usually 1-2 hours.
4. After the reaction is completed, use dialysis or other methods to remove the unreacted BHQ3 Maleimide.
5. Fluorescence imaging or quantitative analysis of proteins is performed using a fluorescence microscope or flow cytometry.
2. Nucleic acid labeling
Experimental steps:
1. Prepare a nucleic acid probe containing thiol.
2. Add BHQ3 Maleimide solution to the nucleic acid solution, and the reaction time is usually 30 minutes to 1 hour.
3. After the reaction is completed, the unreacted probe is removed by purification.
4. Use the labeled nucleic acid probes for PCR amplification, real-time quantitative PCR and other experiments.
5. Use fluorescent instruments to analyze the nucleic acid labeling results as needed.
III. Cell imaging
Experimental steps:
1. Prepare the cell sample and fix it (if necessary).
2. Dissolve BHQ3 Maleimide with a suitable solvent.
3. Add BHQ3 Maleimide to the cell culture medium together with the appropriate fluorescent dye.
4. After incubation for a certain period of time, use a fluorescence microscope to observe the fluorescence images of the cells labeled.