DFHO is a Corn homologous ligand that fluoresces yellow upon excitation and is used to image RNA in living cells.

Corn-DFHO:70 nM (Kd)

I. RNA imaging
1. Aptamer preparation: Corn fluorescent aptamer is combined with target RNA molecules.
2. DFHO binding: DFHO is added to the RNA-aptamer complex. Through its high affinity and fluorescence properties, DFHO will bind to the aptamer and convert to a fluorescent state.
3. Imaging: Through fluorescence microscopy, the fluorescence of DFHO can help locate and quantify the presence of RNA molecules.
4. Optimization: The concentration of DFHO, incubation time, temperature and other conditions can be adjusted to optimize the intensity and stability of the signal.
II. RNA molecular dynamics study
1. Cell treatment: DFHO (1-20uM) and Corn fluorescent aptamer are added to cell culture to label specific RNA in cells.
2. Imaging analysis: Combined with real-time fluorescence microscopy to monitor the changes of RNA in cells, analyze the distribution, transcription and synthesis dynamics of RNA.
III. Gene expression study
1. RNA labeling: Use Corn fluorescent aptamer to label RNA molecules of specific genes.
2. DFHO labeling: Use DFHO to convert RNA signals into visible fluorescent forms.
3. Analysis: Analyze RNA expression at different time points and conditions through fluorescent imaging.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 80 mg/mL (284.48 mM), Sonication is recommended.