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Janelia Fluor® 669, SE

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Catalog No. T39575Cas No. 2127150-20-1
Alias JF669,NHS, JF669, SE, Janelia Fluor? 669, SE

Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, reacts directly with thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to produce a JF669 HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm).

Janelia Fluor® 669, SE

Janelia Fluor® 669, SE

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Catalog No. T39575Alias JF669,NHS, JF669, SE, Janelia Fluor? 669, SECas No. 2127150-20-1
Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, reacts directly with thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to produce a JF669 HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm).
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
2 mg$1,62035 days35 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, reacts directly with thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to produce a JF669 HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm).
In vitro
JF669 rapidly transforms into a thioether product in less than 5 minutes to completion when in organic solvents, primarily assuming the closed form[1]. Additionally, JF669 exhibits superior photostability, maintaining 97% of its fluorescence intensity after an equivalent number of cycles[1].
SynonymsJF669,NHS, JF669, SE, Janelia Fluor? 669, SE
Chemical Properties
Molecular Weight693.77
FormulaC34H30F3N3O6SSi
Cas No.2127150-20-1
Smiles[#6][Si]1([#6])[#6]-2=[#6]\[#6](-[#6]=[#6]-[#6]-2=[#6](-c2ccc(cc12)-[#7]-1-[#6]-[#6]-[#6]-1)-c1c(F)c(-[#16]-[#6]-[#6](=O)-[#8]-[#7]-2-[#6](=O)-[#6]-[#6]-[#6]-2=O)c(F)c(F)c1-[#6](-[#8-])=O)=[#7+]-1/[#6]-[#6]-[#6]-1 |c:6,9,t:3|
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
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g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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Janelia Fluor® 669, SE
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