transfection

DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.

DOPE (DOPE) is a neutral helper lipid for cationic liposomes.It is used in combination with cationic phospholipids to increase efficiency during DNA transfection studies as a non-viral method of gene delivery.

DODAP is an ionic cationic lipid with low cytotoxicity and high transfection efficiency that can be used to synthesize liposomes and encapsulate biologically active molecules such as mRNA, siRNA and plasmid DNA.

DOSPA, a cationic lipid, exhibits significant potential when formulated with DNA as a transfection system [1].

14:1 EPC trifluoromethanesulfonate, an ethyl-phosphatidylcholine (EPC) derivative with monounsaturated 14:1 chains, exhibits transfection activity. This compound is utilized for drug delivery [1] [2].

3'Ome-m7GpppAmpG ammonium, a trinucleotide Cap analogue with a locked nucleic acid (LNA) component, demonstrates substantial translational efficiency. It emerges as a promising tool in molecular biology, applicable in mRNA vaccines and transfection processes including protein production, gene therapy, and anti-cancer immunization [1].

AA-T3A-C12 is an anisamide ligand-tethered lipidoid (AA-lipidoid). AA-T3A-C12 mediates great RNA delivery and transfection of activated fibroblasts [1] .

GL67 (N4-Spermine cholesteryl carbamate), a cationic lipid, facilitates the delivery of nucleic acid agents and vaccines, and is effective in gene transfection [1].

DOTMA (N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium) is a tetra-methylated DOTA analogue. DOTMA is a cationic lipid and can be used as a non-viral vector for gene therapy. It has been used as a component in liposomes that can be used to encapsulate siRNA, microRNAs, and oligonucleotides and for gene transfection in vitro. It exhibits effective in vitro and in vivo gene transfection. DOTMA induces a positive charge on the liposomes and thus promotes efficient liposome- cell membrane interaction.

DMG-PEG2000 (DMG-PEG2000) can be used for the preparation of lipid nanoparticles for oral delivery of plasmid DNA in vivo to improve the mucus permeability and delivery efficiency of nanoparticles. DMG-PEG 2000 can be used for the preparation of siRNA transfected liposomes, which can improve the transfection efficiency.

DOTAP methylsulfat is a cationic lipid reagent derived from trimethylammonium, featuring two 18-carbon fatty acid tails each with a single alkene group. This compound can self-assemble with negatively charged ions (such as DNA) to form complexes that adhere to cell membranes and enter cells through electrostatic interactions and endocytosis. With its hydrophobic domains, DOTAP methylsulfat facilitates the fusion of endosomal membranes to release DNA into the cytoplasm, serving a role in gene delivery. It is widely used in research areas like gene therapy, cell transfection, and the design of non-viral vectors.

GL67 (N4-Spermine cholesteryl carbamate) (pentahydrochloride) is a cationic lipid. It is utilized for the delivery of nucleic acid active molecules, vaccines, and for gene transfection.

DOPE-PEG-DBCO (MW 2000) is a PEG lipid featuring DOPE and DBCO as functional groups. DOPE serves as a helper lipid in gene transfection, PEG is utilized in drug delivery systems, and DBCO is applicable in click chemistry.

Cho-es-Lys is a cationic lipid synthesized from natural cholesterol and amino acids, known for its high gene transfection efficiency. Cho-es-Lys is applicable in drug delivery research.

SAINT-2 is a cationic lipid with gene transfection activity and is a pyridinium lipid analog. It forms molecular membranes that interact with plasmids, creating lipid complexes. Once these complexes are taken up by cells, the plasmid dissociates from the lipid complex due to the action of DOPE, facilitating translocation across the endosomal and/or nuclear membranes. This process efficiently transfers small oligonucleotides into cells.

4-Arm-PEG-DSPE (MW 600) is a PEG crosslinking agent that connects four PEG side chains to a DSPE phospholipid. It is applicable in drug delivery, gene transfection, and vaccine delivery.

4-Arm-PEG-DSPE (MW 10000) is a PEG crosslinking agent that connects four PEG branches with a DSPE phospholipid. This compound is utilized in drug delivery, gene transfection, and vaccine delivery applications.

4-Arm-PEG-DSPE (MW 400) is a PEG crosslinking agent that connects four PEG side chains with a DSPE phospholipid. It is applicable in drug delivery, gene transfection, and vaccine distribution.

4-Arm-PEG-DSPE (MW 5000) is a PEG crosslinking agent that combines four PEG branches with a DSPE phospholipid. It is utilized in drug delivery, gene transfection, and vaccine delivery.

4-Arm-PEG-DSPE (MW 2000) is a PEG cross-linking reagent that connects four PEG arms to a DSPE phospholipid. It is applicable in drug delivery, gene transfection, and vaccine delivery.

4-Arm-PEG-DSPE (MW 3400) is a PEG crosslinking agent that connects four PEG branches to a DSPE phospholipid. It is utilized in drug delivery, gene transfection, and vaccine delivery.

4-Arm-PEG-DSPE (MW 1000) is a PEG crosslinking reagent that links four PEG side chains to a DSPE phospholipid. It is applicable in drug delivery, gene transfection, and vaccine transport.

8-Arm-PEG-DSPE (MW 3400) is a PEG crosslinker that connects PEG side chains to a DSPE phospholipid. It is applicable in drug delivery, gene transfection, and vaccine delivery.

6-Arm-PEG-DSPE (MW 5000) is a PEG crosslinker that connects PEG side chains to a DSPE phospholipid. It is utilized in drug delivery, gene transfection, and vaccine delivery.