transfection

DOPE (DOPE) is a neutral helper lipid for cationic liposomes.It is used in combination with cationic phospholipids to increase efficiency during DNA transfection studies as a non-viral method of gene delivery.

DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.

DODAP is an ionic cationic lipid with low cytotoxicity and high transfection efficiency that can be used to synthesize liposomes and encapsulate biologically active molecules such as mRNA, siRNA and plasmid DNA.

DOSPA, a cationic lipid, exhibits significant potential when formulated with DNA as a transfection system [1].

14:1 EPC trifluoromethanesulfonate, an ethyl-phosphatidylcholine (EPC) derivative with monounsaturated 14:1 chains, exhibits transfection activity. This compound is utilized for drug delivery [1] [2].

3'Ome-m7GpppAmpG ammonium, a trinucleotide Cap analogue with a locked nucleic acid (LNA) component, demonstrates substantial translational efficiency. It emerges as a promising tool in molecular biology, applicable in mRNA vaccines and transfection processes including protein production, gene therapy, and anti-cancer immunization [1].

AA-T3A-C12 is an anisamide ligand-tethered lipidoid (AA-lipidoid). AA-T3A-C12 mediates great RNA delivery and transfection of activated fibroblasts [1] .

GL67 (N4-Spermine cholesteryl carbamate), a cationic lipid, facilitates the delivery of nucleic acid agents and vaccines, and is effective in gene transfection [1].

DOTMA (N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium) is a tetra-methylated DOTA analogue. DOTMA is a cationic lipid and can be used as a non-viral vector for gene therapy. It has been used as a component in liposomes that can be used to encapsulate siRNA, microRNAs, and oligonucleotides and for gene transfection in vitro. It exhibits effective in vitro and in vivo gene transfection. DOTMA induces a positive charge on the liposomes and thus promotes efficient liposome- cell membrane interaction.

DMG-PEG2000 (DMG-PEG2000) can be used for the preparation of lipid nanoparticles for oral delivery of plasmid DNA in vivo to improve the mucus permeability and delivery efficiency of nanoparticles. DMG-PEG 2000 can be used for the preparation of siRNA transfected liposomes, which can improve the transfection efficiency.

DOTAP methylsulfat is a cationic lipid reagent derived from trimethylammonium, featuring two 18-carbon fatty acid tails each with a single alkene group. This compound can self-assemble with negatively charged ions (such as DNA) to form complexes that adhere to cell membranes and enter cells through electrostatic interactions and endocytosis. With its hydrophobic domains, DOTAP methylsulfat facilitates the fusion of endosomal membranes to release DNA into the cytoplasm, serving a role in gene delivery. It is widely used in research areas like gene therapy, cell transfection, and the design of non-viral vectors.

GL67 (N4-Spermine cholesteryl carbamate) (pentahydrochloride) is a cationic lipid. It is utilized for the delivery of nucleic acid active molecules, vaccines, and for gene transfection.

DOPE-PEG-DBCO (MW 2000) is a PEG lipid featuring DOPE and DBCO as functional groups. DOPE serves as a helper lipid in gene transfection, PEG is utilized in drug delivery systems, and DBCO is applicable in click chemistry.

Cho-es-Lys is a cationic lipid synthesized from natural cholesterol and amino acids, known for its high gene transfection efficiency. Cho-es-Lys is applicable in drug delivery research.

Aluminon (ATA; Ammonium aurintricarboxylate; Aurintricarboxylic acid ammonium) functions as a complexometric titration indicator and also acts as a nuclease inhibitor, impacting transfection. Additionally, Aluminon can be utilized as a biomaterial or organic compound in life science research.

DEAE-Dextran (MW 500000) is a complex carbohydrate polymer composed of glucose molecules linked by glycosidic bonds and modified with diethylaminoethyl (DEAE) groups. As a cationic molecule, DEAE-Dextran functions as a transfection agent capable of delivering nucleic acids, such as DNA or RNA, into cells. Its positive charge interacts with negatively charged cell membranes to facilitate nucleic acid uptake. Additionally, DEAE-Dextran serves as an ion exchange chromatography resin, enabling the separation and purification of biomolecules based on their charge.