3,6dichlorotrimellitic acid

3,6-Dichlorotrimellitic acid serves as the fundamental precursor for the synthesis of numerous dichlorinated fluoresceins and rhodamines.

Biotin-11-dUTP [tetra(triethylammonium) salt] [(2S,3S,4R,5R,6R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-6-(hydroxymethyl)-2-[(3aR,4S,6R,6aS)-2-oxohexahydro-2H-thieno[3,4-d]imidazol-4-yl]-tetrahydro-2H-furo[3,2-d][1,3,2]dioxaphosphorin-3-yl]phosphonate] is used as a fluorescent substitute for dTTP.

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.[1] Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.[2] bis-ANS has an excitation maxima of 390 nm.[3] It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.4 It also potently inhibits microtubule assembly.[5],[6]

Coelenterazine hcp is a synthetic bioluminescent luciferin that displays an emission maximum of 445 nm.1It has been used as a calcium indicator and substrate to quantifyRenillaluciferase activity.1,2,3 1.Sabnis, R.W.Handbook of biological dyes and stains: Synthesis and industrial applications(2010) 2.Shimomura, O., Kishi, Y., and Inouye, S.The relative rate of aequorin regeneration from apoaequorin and coelenterazine analoguesBiochemistry Journal296(Pt 3)549-551(1993) 3.Pichler, A., Prior, J.L., and Piwnica-Worms, D.Imaging reversal of multidrug resistance in living mice with bioluminescence: MDR1 P-glycoprotein transports coelenterazineProc. Natl. Acad. Sci. USA.101(6)1702-1707(2004)

1) Non-toxicity: The unique oiliness and high molecular weight characteristics of CelRed prevent it from penetrating cell membranes into cells, and Ames test results also show that the mutagenicity of CelRed dye is much less than EB. 2) High sensitivity:

TNP-ATP, a derivative of ATP, acts as an antagonist to the purinergic P2Y1, P2X3, and P2X2 3 receptors, demonstrating selectivity by showing lower inhibitory concentrations (IC50 = 6, 0.9, 7 nM, respectively) compared to the P2X2, P2X4, and P2X7 receptors (IC50 = 2, 15.2, >30 µM, respectively) in HEK293 cells expressing these human receptors. It effectively diminishes acetic acid-induced calcium influx in 1321N1 cells targeting P2X3 and P2X2 3 receptors (IC50 = 100 and 62 nM, respectively) and alleviates pain in a mouse model of visceral pain (ED50 = 6.35 µmol kg). Moreover, TNP-ATP exhibits a notable fluorescence characteristic, with an emission peak at 547 nm after excitation at 403 nm, which enhances four-fold and shifts to 538 nm when bound to the insulin-degrading enzyme (IDE).

Compound 1a, known as 1-(3',6'-Bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)-3-phenylurea, serves as a fast-responsive fluorescent probe for detecting gold ions in water and synthetic products [1].

Sodium 2,2',2'',2'''-((((1,1-dioxido-3H-benzo[c][1,2]oxathiole-3,3-diyl)bis(6-hydroxy-5-isopropyl-2-methyl-3,1-phenylene))bis(methylene))bis(azanetriyl))tetraacetate is a useful organic compound for research related to life sciences. The catalog number is T65315 and the CAS number is 1945-77-3.

6-Chloro-3-indoxyl-α-D-mannopyranoside serves as a chromogenic substrate for the enzyme α-mannosidase, generating a salmon-colored precipitate upon enzymatic cleavage.

Gallocyanine is a small molecule blue dye used to stain nuclei. It is known to inhibit Dickkopf-1 (Dkk1), an antagonist of the Wnt pathway. Gallocyanine can disrupt the interaction of Dkk1 with its receptor LRP5 6 (IC50 = 3 μM), thereby activating Wnt β-catenin signaling. Modulation of the Dkk1-LRP5 6 interaction via gallocyanine has been shown to inhibit prostaglandin J2-induced tau phosphorylation at serine396 in primary cortical neurons in vitro.

Mca-KPLGL-Dap(Dnp)-AR-NH2 serves as a fluorogenic substrate specifically designed for matrix metalloproteinases (MMPs). This compound facilitates the quantification of MMP activity by releasing 7-methoxycoumarin-4-acetyl (Mca) upon enzymatic cleavage by MMPs, which exhibits fluorescence with excitation and emission maxima at 328 nm and 420 nm, respectively.

ZnAF-2, 6-iso- is a cell-impermeable, high-affinity Zn2-specific fluorescent probe (Kd = 2.7 nM) that remains relatively non-fluorescent until it binds to Zn2 in a stoichiometric (1:1) ratio, resulting in an approximately 51-fold increase in fluorescence intensity. It shows minimal affinity for Ca2, Mg2, Na, or K.