probes

2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA.

3-Cyano-7-hydroxycoumarin (3-Cyanoumbelliferone) is a macrophage inhibitor of macrophage inhibitory factor (MIF) interconjugate isomerase with a Ki of 2.9 μM. 3-Cyano-7-hydroxycoumarin belongs to the group of coumarin derivatives, which can be used as molecular probes and fluorescent dyes.

Fluorescein Sodium (VST-1001) is a phthalic indicator dye. It is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

Dabcyl acid, SE (Dabcyl, SE) is the amino-reactive form of Dabcyl acid and used to prepare a variety of FRET-based probes that contain Dabcyl acid.

ZnAF-1F, with a Kd value of 2.2 nM, serves as a powerful fluorophore and is effective as fluorescent probes for Zn2+ in cellular applications. It exhibits an excitation wavelength (λ excitation) of 489 nm and an emission wavelength (λ emission) of 514 nm [1] [2] [3].

Aminoallyl-UTP (5-(3-Aminoallyl)uridine-5'-O-triphosphate) is an amine-modified nucleotide utilized in affinity-tagged UTP synthesis. Its incorporation into complementary RNA (cRNA) and bacterial transfer-messenger RNA (tmRNA) enables the creation of fluorescently labeled probes for microarrays.

HMRG is a hydroxymethyl rhodamine green fluorescent scaffold featuring an open non-spirocyclic structure. At physiological pH, HMRG undergoes a one-step enzymatic reaction resulting in significant dynamic fluorescence changes, making it suitable for developing various probes. Fluorescent probes based on HMRG can be utilized for detecting diseases such as cancer and diabetic nephropathy.

Acridinium C2 NHS ester is a chemiluminescent label known for its reactivity, making it suitable for developing protein and nucleic acid probes. This compound is widely used in bioanalytical and diagnostic applications, offering high-sensitivity detection solutions. It is particularly valuable in medical research for monitoring and analyzing biomolecules.

Fmoc-protected fluorescent amino acid is designed for solid-phase peptide synthesis, specifically for crafting PAINT imaging probes. It features excitation and emission maxima (λ) at 488 nm and 601 nm, respectively.

Cyamine3.5 NHS ester is a squaraine dye utilized in the development of fluorescent probes and biological labeling, characterized by its intermediate wavelength fluorescence emission properties. Specially designed for reactions with amino groups to form stable covalent bonds, it is ideal for labeling biomolecules. Ex Em(nm)=591 604.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentaammonium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Mgc3Me linker is a connector used for synthesizing mgc3Me fluorescent probes. It modularly attaches the myrGC3Me motif to fluorophores, enabling Golgi apparatus imaging in live cells.

ZVM68437, also known as Mn(III) meso-Tetra(pentafluorophenyl) Porphine Chloride, is a synthetic porphyrin compound. It is primarily utilized for modifying electrode surfaces for cysteine detection and as a component in constructing MRI probes and catalytic oxidation processes. Currently, ZVM68437 does not have an official name and is provisionally assigned this code name according to MedKoo Chemical Nomenclature (refer to the webpage: https: www.medkoo.com page naming).

JF526-Taxol (TFA) serves as a versatile scaffold for fluorogenic probes, including ligands for self-labeling tags, stains for endogenous structures, and spontaneously blinking labels for super-resolution immunofluorescence [1].

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentapotassium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

QSY 21 NHS is an effective energy transfer acceptor for far-red and near-infrared fluorescent probes. This quencher operates within the wavelength range of 540-750 nm, typically utilized in FRET applications. QSY 21 NHS does not emit fluorescence under normal conditions. The NHS ester form of QSY 21 allows for the labeling of proteins, amine-modified oligonucleotides, and other primary amine-containing molecules (R-NH2).

(±)-ANAP is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

BODIPY-FL (BDP FL acid) is a broad-spectrum and effective fluorescent dye that can be used to label probes or primers and is a compound for the quantitative detection of specific DNA RNA based on fluorescence bursts.BODIPY-FL-labelled monoterpenes can be used to detect Gram-positive and Gram-negative bacteria as characteristic and pathogenic fungi.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentaammonium salt is most commonly used among the visible light-excitable calcium indicators.

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe known for its exceptional sensitivity and selectivity in detecting hypochlorous acid. With an absorption wavelength longer than fluorescein-based probes, it offers improved pH stability. Notably, HKOCl-4 exhibits excitation at 530 nm and emission at 557 nm.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentasodium salt is most commonly used among the visible light-excitable calcium indicators.

Quin-2 is a high-affinity fluorescent calcium indicator (Kd = 115 nM for calcium). It displays high selectivity for calcium, as it is not affected by sodium gradients, membrane potential, or intracellular pH. High affinity probes like quin-2 are ideal for monitoring low levels of calcium, as are found in resting cells. Loadings of up to 2 mM quin-2 are without serious toxic effects, so quin-2 may be used to buffer intracellular calcium transients. Excitation emission maxima for quin-2 are 339 and 492 nm, respectively.

ROX NHS ester, 6-isomer, is a highly fluorescent and photostable rhodamine dye used for labeling amino groups in peptides, proteins, and amino-oligonucleotides. ROX-labeled oligonucleotide probes are commonly used in qPCR, and qPCR instruments have a ROX channel. [Pure single isomer.]