firefly luciferase-in-1

Firefly luciferase-IN-1 is a 2-benzylidene tetrahydronaphthone derivative and can be used as a firefly luciferase inhibitor in kit.

Fluc-IN-1 is a potent inhibitor of firefly luciferase (Fluc) (IC 50 = 25 nM) and further inhibits WNT/β-catenin through Fluc (IC50 ≈ 30 nM) without acting through Frizzled 7 or the WNT/β-catenin signaling amplifier.

lucPpy-IN-1 (compound 9) is a potent inhibitor of the ATP-dependent luciferase from Photinus pyralis (lucPpy) with an IC50 value of 4.0 uM. Luciferase enzymes are widely utilized in bioluminescence-based reporter gene assays and high-throughput screening (HTS). lucPpy-IN-1 is primarily used to investigate the inhibitory effects of chemical libraries on firefly luciferase and to assist in assessing the druggability of targets, helping to identify and mitigate false-positive signals in HTS assays.