cinobufagin

Cinobufagin (Cinobufagine) is a selective Na+ K+-ATPase inhibitor. The activity of Cinobufagin is same as ouabain.

3-Oxo-cinobufagin (compound 8), a potential anticancer agent, is extracted from M. spinosus broth using high performance liquid chromatography. This compound is unique among its isolated counterparts because its C-5 hydroxyl group is further oxidized or isomerized, which significantly reduces its cytotoxic activity against most cell lines, except for HEL. However, it demonstrates increased activity in BEL cell strains. The IC50 values of 3-Oxo-cinobufagin for cytotoxicity against cancer cells are: 71.3 μM (HepG2), 90.2 μM (SMMC-7221), 0.11 μM (BEL-7402), 72.5 μM (K562), 5.3 μM (HL-60), and 12 nM (HEL).

1. Telocinobufagin (Telobufotoxin) is the major endogenous digitalis-like factor. 2. Telocinobufagin significantly decreases the bacterial burdens in the spleen and prolongs the survival time of FIST-immunized mice challenged with live S. typhimurium. 3. Telocinobufagin has immunomodulatory activity, can enhance a Th1 immune response to control intracellular infections, could be developed as a novel immunotherapeutic agent to treat cancer and other immune-mediated diseases.

19-Oxocinobufagin is a natural product from Bufo bufo gargarizans Cantor.

Cinobufaginol is a natural product

3-Epicinobufagin (compound IX) serves as a metabolic marker for Bufalin, indicating metabolic status through the detection of its levels in serum and urine.

Desacetylcinobufagin (Deacetylcinobufagin) , a natural product, shows potent activities against Hela and A375 cells in vitro.