antiparasitic agent-22

Antiparasitic agent-22 (Compound 24) is a broad-spectrum antiparasitic compound that inhibits the procyclic form of T. brucei, L. infantum, and L. tropica with IC50 values of 2.41, 5.95, and 8.98 μM, respectively, and the amastigote form of L. infantum with an IC50 of 8.18 μM. It also shows inhibitory activity against the P. falciparum W2 strain with an IC50 of 0.155 μM. Additionally, Antiparasitic agent-22 exhibits low cytotoxicity in THP1 cells, with a CC50 of 64.16 μM.

Antileishmanial agent-22 (compound 15b) acts as an antiparasitic and antibacterial agent, demonstrating activity against Leishmania (IC50 = 0.408 μM), malaria, and tuberculosis. It functions via an antifolate mechanism, inhibiting folic and folinic acids with 88% and 94% efficacy at 100 μM. The compound inhibits P. berghei by 96.67% in vivo/in vitro at 48.4 μM/kg/day and 0.038 μM (IC50), and it inhibits M. tuberculosis with an MIC of 28.44 μM [1].