adenosine 5'-phosphosulfate

Adenosine 5'-phosphosulfate is an ATP and sulfate competitive inhibitor of ATP sulfurylase in humans, S. cerevisiae, and P. chrysogenum (Ki = 18, 2500, and 1500 nM, respectively). Adenosine 5'-phosphosulfate also inhibits human adenosine 5'-phosphosulfate kinase (Ki = 47.5 μM) to prevent sulfation.

Adenosine 3'-phosphate 5'-phosphosulfate lithium serves as a cosubstrate for glycan sulfation. It can be utilized in assays involving Golgi-specific nucleotide phosphohydrolase (adenosine phosphate) 3'-phosphatase coupled with sulfotransferase, acting as a donor substrate for the transfer of the sulfonate group.

Adenosine 3'-phosphate 5'-phosphosulfate lithium is a cosubstrate used in glycoconjugate sulfation processes. It can be applied in specific Golgi phosphoadenosine 3'-phosphatase-coupled sulfotransferase assays, functioning as the donor substrate for transferring sulfate groups.