5 hydroxymethylcytosine

HM-cytosine (5hmC) is a naturally occurring component of mammalian genomic DNA and is thought to be the sixth base of DNA [1]. HM-cytosine is an intermediate metabolite for demethylation of active DNA [2]. HM-cytosine is an ep

NSC-311068 is a novel selective inhibitor of TET1 transcription and 5-hydroxymethylcytosine (5hmC) modification, effectively inhibiting cell viability in AML with high expression of TET1.

Recombinant Ten-Eleven Translocase is a recombinantly expressed dioxygenase that depends on Fe(II) and α-ketoglutarate. It catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC), followed by further oxidation to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Subsequently, 5caC is converted into uracil (U) through chemical or enzymatic processes, and during PCR, uracil (U) is transformed into thymine (T), allowing for single-base resolution and highly accurate identification of DNA methylation sites.

Recombinant APOBEC3A (A3A) Protein is a single-domain cytidine deaminase with antiviral properties that deaminates cytidine into uracil, allowing for specific detection of DNA methylation levels. This protein is recombinantly expressed in Escherichia coli and is suitable for bisulfite-free whole-genome sequencing of 5-hydroxymethylcytosine (e.g., ACE-Seq) and cytidine deamination.

T4 Phage β-glucosyltransferase is a DNA-modifying enzyme encoded by T4 phage, which facilitates the transfer of glucose from uridine diphosphate glucose to the 5-hydroxymethylcytosine bases in phage T4 DNA.