α-galactosidase a

Alpha-Galactosidase A is a lysosomal exoglycosidase that hydrolyzes the terminal α-galactose residues of glycoconjugates.

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside used for detecting α-galactosidase activity. The amount of released pNP increases significantly when PNP-alpha-D-Gal is used as a substrate.

4-Methylumbelliferyl 2-acetamido-2-deoxy-α-D-galactopyranoside is a fluorescent substrate employed to study the enzymatic activity of α-galactosidase. It is utilized for assessing enzyme activity in various biological samples, aiding scientists in understanding the enzyme's role within organisms. Additionally, it serves in screening potential compounds for inhibiting or activating this enzyme.

5-Bromo-4-chloro-1H-indol-3-yl nonanoate is a fluorescent substrate used for enzyme detection. It reacts with various enzymes to produce fluorescent products and has demonstrated activity with β-galactosidase, α-chymotrypsin, and β-glucuronidase.