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4-Nitrophenyl α-D-galactopyranoside

Name: 4-Nitrophenyl α-D-galactopyranoside
Brand: TargetMol
SKU: T19139
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19139Cas No. 7493-95-0

Alias PNP-α-D-Gal, PNP-alpha-D-Gal

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside used for detecting α-galactosidase activity. The amount of released pNP increases significantly when PNP-alpha-D-Gal is used as a substrate.

4-Nitrophenyl α-D-galactopyranoside

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Purity: 99.44%

Catalog No. T19139Alias PNP-α-D-Gal, PNP-alpha-D-GalCas No. 7493-95-0

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 mg	$30	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.44%

Appearance:Solid

Color:White

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside used for detecting α-galactosidase activity. The amount of released pNP increases significantly when PNP-alpha-D-Gal is used as a substrate.
In vitro	Using 4-nitrophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 while no activity was detected at pH 7.0. The digestion assays using pNP glycopyranosides as artificial substrates exhibit that yielding liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.
Synonyms	PNP-α-D-Gal, PNP-alpha-D-Gal

Chemical Properties

Molecular Weight	301.25
Formula	C₁₂H₁₅NO₈
Cas No.	7493-95-0
Smiles	OC[C@H]1O[C@H](Oc2ccc(cc2)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
Relative Density.	1.599 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (165.98 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3195 mL	16.5975 mL	33.1950 mL	165.9751 mL
5 mM	0.6639 mL	3.3195 mL	6.6390 mL	33.1950 mL
10 mM	0.3320 mL	1.6598 mL	3.3195 mL	16.5975 mL
20 mM	0.1660 mL	0.8299 mL	1.6598 mL	8.2988 mL
50 mM	0.0664 mL	0.3320 mL	0.6639 mL	3.3195 mL
100 mM	0.0332 mL	0.1660 mL	0.3320 mL	1.6598 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

α-galactosidaseyieldinsubstratePNP-a-D-GalInhibitorinhibitglycopyranosideartificialacidic-pH4-Nitrophenyl α-D-galactopyranoside4Nitrophenyl αDgalactopyranoside4-Nitrophenyl alpha-D-galactopyranoside4-Nitrophenyl a-D-galactopyranoside4-nitrophenyl4 Nitrophenyl α D galactopyranoside

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