Energy Metabolism Kit

Glucose-6-phosphate (6PG) is an intermediate product of glycolysis and the pentose phosphate pathway, widely existing in animals, plants, and microorganisms. In the first step of glycolysis, glucose is catalyzed by hexokinase to form glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to proceed with other steps of glycolysis. In the pentose phosphate pathway, glucose-6-phosphate is its first substrate, and this process is also the main pathway for NADPH generation. In addition, glucose-6-phosphate can be converted into glycogen or starch for storage.

Aconitase (ACO) is an enzyme in the tricarboxylic acid cycle that catalyzes the conversion of citric acid to isocitric acid. Citric acid itself is not easily oxidized. Under the action of aconitase, through dehydration and hydration reactions, the hydroxyl group is transferred from the β-carbon atom to the α-carbon atom, generating isocitric acid which is easy to be dehydrogenated and oxidized, preparing for further oxidative decarboxylation reactions.

Acetyl-CoA widely exists in animals, plants, microorganisms and cultured cells, and it is an important intermediate metabolite produced during the metabolism of energy substances in organisms. It is a pivotal substance in the metabolism of energy substances in the body. The three major nutrients, namely carbohydrates, fats and proteins, converge through acetyl-CoA into a common metabolic pathway - the tricarboxylic acid cycle and oxidative phosphorylation, through which they are completely oxidized to carbon dioxide and water, releasing energy for ATP synthesis. In addition, acetyl-CoA is a precursor substance for the synthesis of physiological active substances such as fatty acids, ketone bodies, cholesterol and their derivatives.

Aconitase (ACO) is an enzyme in the tricarboxylic acid cycle that catalyzes the conversion of citric acid to isocitric acid. Citric acid itself is not easily oxidized. Under the action of aconitase, through dehydration and hydration reactions, the hydroxyl group is transferred from the β-carbon atom to the α-carbon atom, generating isocitric acid which is easy to be dehydrogenated and oxidized, preparing for further oxidative decarboxylation reactions.

Acetyl-CoA widely exists in animals, plants, microorganisms and cultured cells, and it is an important intermediate metabolite produced during the metabolism of energy substances in organisms. It is a pivotal substance in the metabolism of energy substances in the body. The three major nutrients, namely carbohydrates, fats and proteins, converge through acetyl-CoA into a common metabolic pathway - the tricarboxylic acid cycle and oxidative phosphorylation, through which they are completely oxidized to carbon dioxide and water, releasing energy for ATP synthesis. In addition, acetyl-CoA is a precursor substance for the synthesis of physiological active substances such as fatty acids, ketone bodies, cholesterol and their derivatives.

Acetate kinase (ACK) mainly exists in microorganisms and catalyzes the conversion of acetate and ATP into acetyl phosphate and ADP. It is a key enzyme in bacterial carbon metabolism and energy metabolism, and plays a central role especially in the methanogenic metabolism of archaea.

Glucose-6-phosphate (6PG) is an intermediate product of glycolysis and the pentose phosphate pathway, widely existing in animals, plants, and microorganisms. In the first step of glycolysis, glucose is catalyzed by hexokinase to form glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to proceed with other steps of glycolysis. In the pentose phosphate pathway, glucose-6-phosphate is its first substrate, and this process is also the main pathway for NADPH generation. In addition, glucose-6-phosphate can be converted into glycogen or starch for storage.

Acetate kinase (ACK) mainly exists in microorganisms and catalyzes the conversion of acetate and ATP into acetyl phosphate and ADP. It is a key enzyme in bacterial carbon metabolism and energy metabolism, and plays a central role especially in the methanogenic metabolism of archaea.