ptdins-(3,4,5)-p3-biotin

The PtdIn phosphates play an important role in the generation and transduction of intracellular signals. PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.

PtdIns-(4,5)-P2-biotin trisodium is a biotin-labeled analog of PtdIns-(4,5)-P2. It serves as an affinity probe for PI3Kγ, which catalyzes the phosphorylation of PtdIns-(4,5)-P2 at the 3′-OH group, producing the second messenger PtdIns-(3,4,5)-P3.