permeabilized

Cy5-DBCO is a near-infrared, anthocyanine fluorescent dye Cy5 (Cyanine 5) derivative with an excitation wavelength of 650 nm and an emission wavelength of 670 nm.DBCO-Sulfo-Cy5 contains a DBCO moiety, which can undergo SPAAC click chemistry with molecules that contain Azide moieties.

BP Fluor 430 DBCO reacts with azides through a copper-free click chemistry reaction to create a stable triazole, eliminating the need for a Cu catalyst or high temperatures. In applications where copper presence is a concern, BP Fluor 430 DBCO serves as an ideal substitute for copper in fluorescent alkyne requirements. BP Fluor 430 is a bright, photostable green fluorescent probe, optimally excited near its absorption maximum at 432 nm. Its emission peak at 539 nm remains unaffected across a broad pH range. Due to high background interference, BP Fluor DBCO reagents are unsuitable for staining intracellular components of fixed and permeabilized cells.

Phalloidin-AMCA conjugate is a blue fluorophore that specifically labels filamentous actin (F-actin). It consists of aminomethylcoumarin (AMCA; ex em max = 350 450 nm) conjugated with phalloidin , a mycotoxin that binds F-actin. Phalloidin-AMCA conjugate is used in fixed and permeabilized tissue sections, cell cultures, or cell-free experiments.