bcip

BCIP (BCIP p-toluidine salt) (BCIP p-toluidine salt) is an artificial chromogenic substrate. It is used for the sensitive colorimetric detection of alkaline phosphatase activity.

BCIP (dipotassium) is a versatile dye utilized in biological experiments to observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Its applications extend from fundamental scientific research to a broad spectrum of clinical diagnostics, as well as traditional fields like textile dyeing and emerging areas such as functional textile processing, food pigments, and dye-sensitized solar cells.

Nitro blue tetrazolium chloride (NBT), an NADPH-diaphorase substrate (IC50 = 3-4 M) and well-known scavenger of superoxide anions, competitively inhibits nitric oxide synthase. Additionally, NBT, as a substrate for dehydrogenases, is used with the alkaline phosphatase substrate BCIP in western blotting and immunohistological staining procedures.