6,7-diethoxy-4-methylcoumarin

6,7-Diethoxy-4-methylcoumarin, a fluorogenic substrate and a metabolite of Cordyceps militaris [1].

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one (Compound 1) serves as a fluorescent probe for detecting hydrogen sulfide (H2S). It is characterized by a low detection limit (4×10^-6 mol/L), high selectivity, and great sensitivity. At a concentration of 150 µg/mL, it exhibits negligible cytotoxicity. The excitation wavelength is 331 nm, while in DMSO solvent, it emits at approximately 385 nm; upon addition of HS-, the fluorescence intensity significantly increases around 392 nm.

6, 8-Difluoro-7-hydroxy-4-methylcoumarin NHS ester is a molecule with an excitation wavelength of 365 nm and an emission wavelength of 460 nm. The NHS ester can be used to label primary amines (-NH2) on proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Ac-ANW-AMC is a fluorogenic substrate for the β5i/LMP7 subunit of the 20S immunoproteasome. [1] Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released, and its fluorescence quantifies the activity of the β5i/LMP7 subunit. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

NBD-X acid is an environmentally sensitive fluorescent probe used to study biological polymers such as lipids and proteins, with a higher labelling yield than NBD and significantly reduced fluorescence intensity in aqueous solutions. NBD-X acid emits green to yellow fluorescence with excitation/absorption wavelengths of 465/535 nm.