4-thiouridine

4-Thiouridine (4-SU) is a photoactivatable ribonucleoside analog that is widely used for RNA analysis, including short-range RNA-RNA crosslinking and nascent RNA labeling. The crosslinking thio moiety is attached directly to the nucleotide base, thus 4-SU

4-Thiouridine 5′-triphosphate tetralithium (4-Thio-UTP), a potent P2Y2 and P2Y4 receptor agonist, exhibits EC50 values of 35 nM for hP2Y2 and 350 nM for hP2Y4. It is utilized in crosslinking experiments and labeling of transcriptional complexes [1] [2].

4-Thiouridine 5′-triphosphate (4-Thio-UTP) tetrasodium functions as a potent agonist at P2Y 2 and P2Y 4 receptors, with half-maximal effective concentrations (EC50s) of 35 nM for hP2Y 2 and 350 nM for hP2Y 4. This compound is applicable in studies involving crosslinking experiments and labeling of the transcriptional complex [1] [2].

5-Methyl-4-thiouridine is a nucleoside derivative belonging to the thio-nucleosides and categorized as a 5-modified pyrimidine nucleoside [5-Methyl-4-thiouridine].

2'-O-Methyl-4-thiouridine is a Nucleoside Derivative - Thio-nucleoside, 2'-Modified nucleoside.

2'-beta-C-Methyl-4-thiouridine is a Nucleoside Derivative - Thio-nucleoside, 2'-Modified nucleoside.

5-Ethyl-4-thiouridine is a nucleoside derivative, specifically a thio-nucleoside, characterized as a 5-modified pyrimidine nucleoside.

5-Methoxy-4-thiouridine is a nucleoside derivative specifically classified as a thio-nucleoside and a 5-modified pyrimidine nucleoside.

2'-Deoxy-4-thiouridine is a Nucleoside Derivative - Thio-nucleoside.

2',3',5'-Tri-O-benzoyl-4-thiouridine is a Nucleoside Derivative - Thio-nucleoside.

Nucleoside Derivatives - Thio-nucleosides, Fluoro-Modified nucleosides, 2'-Modified nucleosides

Nucleoside Derivatives - Thio-nucleosides, 2'-Modified nucleosides, 5-Modified pyrimidine nucleosides

4'-Thiouridine is a Thio-nucleoside.

5-Methyl-4'-thiouridine is a Thio-nucleoside.

Nucleoside Derivatives - Thio-nucleosides; 5-Modified pyrimidine nucleosides

Nucleoside Derivatives - 5-Modified pyrimidine nucleosides; Thio-nucleosides; Fluoro-modified nucleosides

Nsp12-IN-2 (Compound 8), a triphosphate metabolite of 4'-thiouridine, functions as an inhibitor of SARS-CoV-2 Nsp12. It effectively suppresses the RNA-dependent RNA polymerase (RdRp) activity of the SARS-CoV-2 Nsp12-Nsp7-Nsp8 complex, halting RNA synthesis and blocking the RNA capping and NMP capping of Nsp9. Nsp12-IN-2 shows potential for research into infections caused by SARS-CoV-2, other coronaviruses, and additional RNA viruses.

5'-O-DMT-Ri can be used in the synthesis of oligoribonucleotides[1]. [1]. Kadokura M. Synthesis of 4-thiouridine, 6-thioinosine, and 6-thioguanosine 3',5'-O-bisphosphates as donor molecules for RNA ligation and their application to the synthesis of photoactivatable TMG-capped U1 snRNA fragments. J Org Chem. 2000 Aug 25;65(17):5104-13.

4-Thiouracil is a photoactivatable probe designated for site-specific applications in detecting RNA structures and nucleic acid-nucleic acid contacts. Upon illumination with ultraviolet light exceeding 300 nm and in the presence of oxygen, it serves as an energy donor, facilitating the generation of singlet oxygen through triplet-triplet energy transfer. This process enables the highly reactive oxygen species to interact with 4-thiouracil, leading to the formation of uracil and uracil-6-sulfonate; the latter exhibits fluorescence around a wavelength of approximately 390 nm. Additionally, 4-Thiouracil functions as a substrate for T. gondii uracil phosphoribosyltransferase, allowing the synthesis of 4-thiouridine monophosphate for subsequent integration into RNA.

2',3',5'-Tri-O-Benzoyl-4'-thiouridine is a Thio-nucleoside.

MTSEA-biotin is a biotin-conjugated 2-aminoethyl methanethiosulfonate used for the specific labeling and modification of nucleosides. It binds to 4-thiouridine (s4U) and is utilized for visualizing newly transcribed tRNA in Saccharomyces cerevisiae cells.