4-methylumbelliferyl-β-d-glucopyranoside

4-Methylumbelliferyl-β-D-Glucopyranoside (4-MU-GLU) is used in the GCase activity assay based on the catalytic hydrolysis of 4-methylumbelliferyl β-D-glucopyranoside that releases the highly fluorescent 4-methylumbelliferyl (4-MU).

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside is a fluorogenic substrate for β-hexosaminidases that releases fluorescent 4-methylumbelliferone upon enzymatic cleavage, enabling sensitive quantification of enzyme activity across pH-dependent excitation and emission ranges, and is extensively used in clinical and research assays for GM2 gangliosidoses such as Tay-Sachs disease.

β-Xylosidase, derived from Opitutus terrae (EC 3.2.1.37), releases reducing sugars from birchwood xylan and also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. Notably, β-Xylosidase lacks endoxylanase, arabinoxylanase, or β-glucanase activities. Post-translational protein glycosylation occurs in β-Xylosidase.

β-Xylosidase, derived from Lactobacillus brevis (EC 3.2.1.37), is capable of releasing reducing sugars from birchwood xylan and catalyzing the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. It lacks endo-xylanase, arabinoxylanase, or β-glucanase activity. Post-translational modifications of β-xylosidase include glycosylation.

β-Xylosidase (thermostable) releases reducing sugars from birchwood xylan and catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. It lacks endoxylanase, arabinoxylanase, and β-glucanase activity. Post-translational glycosylation modifies β-Xylosidase.

4-Methylumbelliferyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside is a reagent used in biochemical reactions.

4-Methylumbelliferyl 2-trifluoroacetyl-3,4,6-O-triacetyl-2-deoxy-β-D-glucopyranoside is a reagent used in biochemical reactions.