indo-1

Indo-1 (potassium salt) is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when Indo-1 (potassium salt) binds calcium. Indo-1 (potassium salt) is prone to photobleaching, which limits its usefulness in methods involving microscopy.

Indo-1 (sodium salt) is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when Indo-1 (sodium salt) binds calcium. Indo-1 (sodium salt) is prone to photobleaching, which limits its usefulness in methods involving microscopy.

Indo-1 AM is an indicator of fluorescent Ca2+ (λex=340 nm, λem=405 485 nm).

Mag-Indo-1 tetrapotassium salt is a cell-impermeable fluorescent probe designed for detecting Mg2+ [1].

Mag-indo-1 AM, a cell-permeable fluorescent indicator for Mg2+ and Ca2+ (Ex=340-390 nm, Em=410-490 nm), is used to determine intracellular Mg2+ and Ca2+ concentrations [1][2].

Rhod-2 (potassium salt) is a water-soluble, red fluorescent calcium indicator that exhibits a significant shift in fluorescence intensity upon calcium binding (ex max = 549 nm; calcium-free v. ex em max = 552 581 nm; calcium-bound). Unlike the UV-excitable indicators fura-2 and indo-1, it does not demonstrate a spectral shift.

Rhod-2 (sodium salt) is a water-soluble, red fluorescent calcium indicator. It exhibits a significant shift in fluorescence intensity upon calcium binding (ex max = 549 nm; calcium-free v. ex em max = 552 581 nm; calcium-bound). [1][2] Unlike the UV-excitable indicators fura-2 and indo-1 , there is no accompanying spectral shift.