IDO1 Protein, Human, Recombinant (His)

Name: IDO1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01821
Rating: 4.5 (1 reviews)

(Synonyms: indoleamine 2,3-dioxygenase 1, Indoleamine 2,3‑dioxygenase, INDO, IDO-1, IDO) Copy Product Info

Synonyms: indoleamine 2,3-dioxygenase 1, Indoleamine 2,3‑dioxygenase, INDO, IDO-1, IDO

Catalog No. TMPY-01821 Copy Product Info

IDO Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 46 kDa and the accession number is P14902-1.

Pack Size	Price	USA Stock	Global Stock
10 µg	$152	7-10 days	7-10 days
20 µg	$248	7-10 days	7-10 days
50 µg	$577	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to oxidize L-tryptophan to N-formylk-ynurenine. The specific activity is > 400 pmoles/min/μg.
Description	IDO Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 46 kDa and the accession number is P14902-1.
Species	Human
Expression System	E. coli
Tag	N-His
Accession Number	P14902-1
Construction	A DNA sequence encoding the human IDO1 (P14902-1) (Ala 2-Gly 403) was expressed, with a polyhistide tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 88 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 50 mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	indoleamine 2,3-dioxygenase 1, Indoleamine 2,3‑dioxygenase, INDO, IDO-1, IDO
Research Background	Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1, and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 27, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrole ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: ELISA AntibodiesImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

46 kDa (predicted); 44 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.