7acc-2

7ACC2 is a new potent MCT inhibitor with IC50 of 11 nM for [14C]-lactate influx; new antitumor treatment targeting lactate transport in Y cells.

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress.

H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex Em=488 525 nm) with cell membrane permeability.

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

1-Methyl-7-nitroisatoic anhydride (1M7) is a reagent. It detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.

FHZ is a fluorescent probe (IUPAC: 2-(7-hydroxy-3H-phenoxazin-3-one-10-yl)benzoic acid), widely utilized in biological imaging due to its high sensitivity and selectivity in detecting specific molecular targets within cellular environments, exhibiting optimal excitation and emission spectra for various fluorescence microscopy applications.

BCECF is a pH-sensitive fluorescent dye and a cytosolic pH indicator. It can be used for detecting intracellular pH levels.

Compound 2f, chemically known as 4-Methyl-7-(2-nitrophenoxy)-2H-chromen-2-one, is a selenium alcohol fluorescent probe designed on the nucleophilic aromatic substitution mechanism. It exhibits selective recognition of selenols in neutral aqueous solutions without significant interference from biological thiols, amines, or alcohols. This compound is utilized for quantifying selenium content in selenoproteins and imaging the activity of endogenous selenols in living cells.

7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one (Compound 1) serves as a fluorescent probe for detecting hydrogen sulfide (H2S). It is characterized by a low detection limit (4×10^-6 mol L), high selectivity, and great sensitivity. At a concentration of 150 µg mL, it exhibits negligible cytotoxicity. The excitation wavelength is 331 nm, while in DMSO solvent, it emits at approximately 385 nm; upon addition of HS-, the fluorescence intensity significantly increases around 392 nm.

4-Methyl-7-(4-nitrophenoxy)-2H-chromen-2-one is a fluorescent coumarin derivative with excitation and emission wavelengths of 323 nm and 445 nm, respectively.

STY-BODIPY is a styrene-conjugated fluorogenic probe for radical-trapping antioxidant (RTA) activity.1 Co-autoxidation of the STY-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring the loss of absorbance at 571 nm. STY-BODIPY has been used to measure the activity of RTAs, as well as the kinetics and stoichiometry of RTA reactions in cell-free assays.1,2,3References1. Haidasz, E.A., Van Kessel, A.T.M., and Pratt, D.A. A continuous visible light spectrophotometric approach to accurately determine the reactivity of radical-trapping antioxidants. J. Org. Chem. 81(3), 737-744 (2016).2. Shah, R., Shchepinov, M.S., and Pratt, D.A. Resolving the role of lipoxygenases in the initiation and execution of ferroptosis. ACS Cent. Sci. 4(3), 387-396 (2018).3. Chauvin, J.-P.R., Haidasz, E.A., Griesser, M., et al. Polysulfide-1-oxides react with peroxyl radicals as quickly as hindered phenolic antioxidants and do so by a surprising concerted homolytic substitution. Chem. Sci. 7(10), 6347-6356 (2016). STY-BODIPY is a styrene-conjugated fluorogenic probe for radical-trapping antioxidant (RTA) activity.1 Co-autoxidation of the STY-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring the loss of absorbance at 571 nm. STY-BODIPY has been used to measure the activity of RTAs, as well as the kinetics and stoichiometry of RTA reactions in cell-free assays.1,2,3 References1. Haidasz, E.A., Van Kessel, A.T.M., and Pratt, D.A. A continuous visible light spectrophotometric approach to accurately determine the reactivity of radical-trapping antioxidants. J. Org. Chem. 81(3), 737-744 (2016).2. Shah, R., Shchepinov, M.S., and Pratt, D.A. Resolving the role of lipoxygenases in the initiation and execution of ferroptosis. ACS Cent. Sci. 4(3), 387-396 (2018).3. Chauvin, J.-P.R., Haidasz, E.A., Griesser, M., et al. Polysulfide-1-oxides react with peroxyl radicals as quickly as hindered phenolic antioxidants and do so by a surprising concerted homolytic substitution. Chem. Sci. 7(10), 6347-6356 (2016).

7-Azido-4-methylcoumarin (7-azido-4-methyl-2H-chromen-2-one) is a fluorescent photoactive probe used in the study of human phenol sulfotransferase (SULT1A1 or P-PST-1) substrate binding sites through photoaffinity labeling.

7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation emission maxima of 380 515 nm, respectively.1 1.Imai, K., Toyo'oka, T., and Watanabe, Y.A novel fluorogenic reagent for thiols: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonateAnal. Biochem.128(2)471-473(1983) 2.Ichinose, S., Nakamura, M., Maeda, M., et al.A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasmaBiomed. Chromatogr.23(9)935-939(2009)

Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively. 1.Zimmerman, M., Ashe, B., Yurewicz, E.C., et al.Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substratesAnal. Biochem.78(1)47-51(1977) 2.Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al.Proteolytic degradation of host hemoglobin by schistosomesMol. Biochem. Parasitol.89(1)1-9(1997)

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O'Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

2-(7-Methoxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T64951 and the CAS number is 62935-72-2.

2-(7-Hydroxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T65277 and the CAS number is 6950-82-9.

7-(Diethylamino)-4-methyl-2H-chromen-2-one is a useful organic compound for research related to life sciences. The catalog number is T66262 and the CAS number is 91-44-1.

Acid orange 7 (COLIPA C015) is a dye used to dye wool.

7-Hydroxy-4-methyl-2(1H)-quinolone (compound 2b), a fluorescent hydroxylated derivative, serves as a probe for detecting hydroxyl radicals associated with DNA damage [1]. It should be protected from light for preservation.

4-(2-Aminoethylamino)-7-(N,N-dimethylsulfamoyl)benzofurazan functions as a precolumn fluorescence derivatization reagent.

TNP-ATP, a derivative of ATP, acts as an antagonist to the purinergic P2Y1, P2X3, and P2X2 3 receptors, demonstrating selectivity by showing lower inhibitory concentrations (IC50 = 6, 0.9, 7 nM, respectively) compared to the P2X2, P2X4, and P2X7 receptors (IC50 = 2, 15.2, >30 µM, respectively) in HEK293 cells expressing these human receptors. It effectively diminishes acetic acid-induced calcium influx in 1321N1 cells targeting P2X3 and P2X2 3 receptors (IC50 = 100 and 62 nM, respectively) and alleviates pain in a mouse model of visceral pain (ED50 = 6.35 µmol kg). Moreover, TNP-ATP exhibits a notable fluorescence characteristic, with an emission peak at 547 nm after excitation at 403 nm, which enhances four-fold and shifts to 538 nm when bound to the insulin-degrading enzyme (IDE).

Compound 1a, known as 1-(3',6'-Bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)-3-phenylurea, serves as a fast-responsive fluorescent probe for detecting gold ions in water and synthetic products [1].

2,7-Dichlorodihydrofluorescein (DCFH) is a non-fluorescent probe that is oxidized by intracellular reactive oxygen species (ROS) to produce the highly fluorescent 2',7'-Dichlorofluorescein (DCF).DCFH is commonly used to assay the intracellular level of ROS, to detect ROS production and to analyze oxidative stress.