3,3',5,5'-tetramethylbenzidine

TMB dihydrochloride (Sure Blue TMB dihydrochloride) is an enzyme-linked immunosorbent assays (ELISA) and an excellent colorimetric substrate for the detection of horseradish peroxidase (HRP)-labeled probes used in staining procedures immunohistochemistry.

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside (X-Glc) is a chromogenic substrate for β-galactosidase, producing a blue product under β-galactosidase catalysis, commonly used for in situ β-galactosidase staining and blue-white screening.

5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside is a chromogenic compound used to detect cellobiohydrolases.

X-Gluc Dicyclohexylamine (5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt) is a reagent to detect β-glucuronidase and can be used in molecular biology experiments to mark and select the expression of target genes.

Alexa fluor 647 maleimide is a water-soluble fluorescent dye that maintains stability under a broad range of physiological conditions. Alexa fluor 647 maleimide facilitates the covalent attachment of fluorescent groups to proteins through selective reaction with thiol groups, thereby enabling precise labeling. This labeling method offers both high sensitivity and specificity, allowing detection of proteins at very low concentrations. Alexa fluor 647 maleimide is widely applied in the study of protein expression and localization, and it can also be conjugated to antibodies for labeling cell surface antigens or specific proteins within tissues [Ex/Em(nm)=651/671].

8-(4-ethynylphenyl)-1,3,5,7-tetramethyl BODIPY is an organic fluorescent dye characterized by an ethynylphenyl group at the 8-position of the boron dipyrromethene (BODIPY) structure and methyl substituents at the 1, 3, 5, and 7 positions. It boasts a high fluorescence quantum yield and specific chemical properties, making it useful for applications such as fluorescence imaging.

8-(4-bromophenyl)-1,3,5,7-tetramethyl-BODIPY is a derivative based on the BODIPY structure, featuring four methyl groups at positions 1, 3, 5, and 7, with a para-bromophenyl group attached at position 8. This compound finds extensive use in fields such as bioimaging, chemical analysis, and materials science, serving as a fluorescent probe for imaging biological samples, detecting specific chemicals, or in the development of optical materials.

5-FAM-NH2.HCl is a derivative fluorescent dye of 5-FAM. 5-FAM (5-Carboxyfluorescein) is a green fluorescent probe and fluorescein derivative used for labelling peptides, proteins, and nucleic acids, with excitation/emission wavelengths of 490/520 nm.

Cy3-N3,sulfo Cy3(Et)-N3.DIPEA is a CY3-derived dye. CY3 (Sulfo-Cyanine3) is an amine-reactive cyanine orange dye with an Ex/Em of 550/570 nm, commonly used for fluorescent labelling of proteins and nucleic acids.

Cy5-PEG3-Tetrazin is a derivative of Cyanine 5 (Cy5) dye comprising three PEG units. It features a Tetrazine group that can engage in an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing a TCO group.

Cy5-PEG3-TCO is a Cy5 dye derivative containing three PEG units. The TCO group in Cy5-PEG3-TCO enables it to undergo an inverse electron-demand Diels-Alder reaction (iEDDA) with molecules bearing a Tetrazine group.

Cy5-PEG3-SCO is a Cyanine 5 (Cy5) dye derivative containing three PEG units. The compound features an SCO group that enables covalent bonding with amino groups. Typically, SCO is utilized for reactions with amino acid residues of proteins or peptides, especially lysine.

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.[1] Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.[2] bis-ANS has an excitation maxima of 390 nm.[3] It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.4 It also potently inhibits microtubule assembly.[5],[6]

Cal Green 1 is a cell-impermeant fluorescent calcium indicator that is characterized by high quantum yield and low phototoxicity. Its peak excitation and emission wavelengths (506 and 531 nm, respectively) are comparable to standard fluorescein dyes, making Cal Green 1 appropriate for fluorescent microscopy. Cal Green 1 is ~5-fold brighter than fluo-3 at saturating calcium levels.

The rapid, selective, and sensitive sensing of thiols is important in diverse areas of research. This thiofluor 623 responds upon exposure to thiols with an increase in fluorescence intensity of up to 120-fold. The response is selective for thiols and occurs in aqueous media. In the absence of thiols, the probe is essentially non-fluorescent; thiols cause cleavage of the probe, generating a fluorophore with an absorption maximum of 563 nm and emission at 623 nm. The fluorescence quantum yield of the cleaved product, generated in response to thiols, increases in more viscous media, suggesting ideal performance in biological systems and applicability to single-molecule or 2-photon sensing schemes. The thiofluor 623 is cell-permeable and reacts selectively with intracellular thiols. The pseudo-first order rate constant (kobs) depends on substrate (e.g., 2.1 x 10-3 s-1 for cysteine, 2.0 x 10-5 s-1 for human serum albumin).

Biotin-11-dUTP [tetra(triethylammonium) salt] [(2S,3S,4R,5R,6R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-6-(hydroxymethyl)-2-[(3aR,4S,6R,6aS)-2-oxohexahydro-2H-thieno[3,4-d]imidazol-4-yl]-tetrahydro-2H-furo[3,2-d][1,3,2]dioxaphosphorin-3-yl]phosphonate] is used as a fluorescent substitute for dTTP.

Sodium 2,6-dibromo-4-(3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-2,2-dioxido-3H-benzo[d][1,2]oxathiol-3-yl)-3-methylphenolate is a useful organic compound for research related to life sciences. The catalog number is T64788 and the CAS number is 62625-32-5.

5-Bromo-4-chloro-3-indolyl-b-D-glucuronide sodium salt is a useful organic compound for research related to life sciences. The catalog number is T64896 and the CAS number is 129541-41-9.

Sodium 2,2',2'',2'''-((((1,1-dioxido-3H-benzo[c][1,2]oxathiole-3,3-diyl)bis(6-hydroxy-5-isopropyl-2-methyl-3,1-phenylene))bis(methylene))bis(azanetriyl))tetraacetate is a useful organic compound for research related to life sciences. The catalog number is T65315 and the CAS number is 1945-77-3.

Sodium 4-(3-(4-hydroxy-5-isopropyl-2-methylphenyl)-1,1-dioxido-3H-benzo[c][1,2]oxathiol-3-yl)-2-isopropyl-5-methylphenolate is a useful organic compound for research related to life sciences. The catalog number is T66669 and the CAS number is 62625-21-2.

7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is a useful organic compound for research related to life sciences. The catalog number is T67683 and the CAS number is 96735-88-5.

HMBR are not fluorescent by themselves, but when combined with Y-FAST, they emit yellow light when excited by blue light.

5-Dodecanoylaminofluorescein, a lipophilic fluorescent probe conjugated with a free fatty acid derived from fluorescein, is used to analyze membrane fluidity and assess the critical micelle concentration of detergents. Additionally, it serves as a precursor in the synthesis of hydrophobic nanospheres suitable for drug delivery [1] [2] [3].

Fmoc-Lys(5-FITC)-OH is a fluorescent probe used to detect amine labelling of peptides or proteins and can serve as a building block for organic synthesis.