sgc

Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM).

SGC-CK2-2 is an innovative and potent cell-active probe capable of inhibiting CK2 CSNK2.

TNP-GTP, a fluorescent derivative of guanosine 5'-triphosphate (GTP), essential for protein synthesis and gluconeogenesis, exhibits an emission peak at 552 nm when excited at 410 nm in water. Its fluorescence intensity increases and emission peaks shift to 544 nm and 532 nm in 40% and 80% N,N-dimethylformamide, respectively, due to the solvent's lower polarity compared to water. As a potent inhibitor of glutamate dehydrogenase (Ki = 2.7 μM), TNP-GTP enhances fluorescence and shifts its emission to 545 nm upon binding to this enzyme, a process reversible by GTP addition. Additionally, it serves as an antagonist to purinergic P2X2 and P2X2 3 receptors (IC50s = 0.4 and 1.2 nM, respectively) and selectively inhibits rat soluble guanylyl cyclase (sGC; Ki = 11 nM) over bovine liver glutamate dehydrogenase (GDH; Ki = 2.7 µM) and the calmodulin-dependent B. pertussis adenylyl cyclase (AC) toxin, with inhibition constants of 20 µM and 320 µM in the presence of manganese and magnesium, respectively.