Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM).

Tau filament:1.9μM

Instructions
1. Cell and tissue staining
1. Dissolve methylene blue in an appropriate solvent (such as water or buffer) to prepare a dye solution of a certain concentration.
2. Soak the cells or tissue sections in the dye solution, usually the staining time is several minutes.
3. After cleaning, observe the cell structure or distribution of target molecules under a microscope.
2. As MAO-A inhibitor and antidepressant and anti-anxiety drugs
Methods: Through animal models or cell culture systems, the regulatory effect on neurotransmitters was studied.
III. Research on neurodegenerative diseases
Methods: Through animal models and cell models, its neuroprotective effects were studied.