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Methylene Blue trihydrate

Name: Methylene Blue trihydrate
Brand: TargetMol
SKU: T0006
Price: 36 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0006Cas No. 7220-79-3

Alias Methylthionine chloride, Methylene Blue, Basic blue 9

Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM).

Methylene Blue trihydrate

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Purity: 99.6%

Catalog No. T0006Alias Methylthionine chloride, Methylene Blue, Basic blue 9Cas No. 7220-79-3

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 g	$36	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.6%

Color:Green to Black

Resource Download

COA LCMS HNMR

Product Introduction

Methylene Blue trihydrate AI Summary

Methylene Blue trihydrate has a high solubility of 53.4 μM in a 100 mM phosphate buffer at pH 7.0, suggesting it is readily dissolved and available for biological activities in aqueous environments. The compound displays a spectrum of bioactivities, including cytotoxic effects on various cell lines, affecting cell viability and ATP levels. It inhibits Setaria cervi glutathione reductase with a 24% inhibition rate at concentrations between 2 to 200 μM. Additionally, it demonstrates inhibitory effects on pathways and enzymes related to DNA, hormone receptors, genomic imprints, and metabolic activity. Methylene Blue trihydrate exhibits significant antiviral activity against SARS-CoV-2, achieving a 99.16% inhibition rate at 10 μM concentration after 48 hours. It has a selectivity index of 4.0 for SARS-CoV-2 induced cytotoxicity in Caco-2 cells with an IC50 value of 2030.0 nM and a CC50 value of 7250.0 nM, suggesting a favorable safety profile. It inhibits native rhodanese and Escherichia coli GroEL with IC50 values of 6100.0 nM and 3300.0 nM, respectively, and inhibits ATPase activity of E. coli GroEL with an IC50 >250000.0 nM. Moreover, it shows 67% and 54% inhibition against the refolding activity of E. coli GroEL for rhodanese and pig heart malate dehydrogenase (MDH), respectively. Despite its broad antiviral and enzyme inhibitory activities, Methylene Blue trihydrate demonstrates inconsistent antiviral effects against SARS-CoV-2 3CL-Pro protease, with an inhibition percentage of -9.023% at a 20 μM concentration, indicating variable efficacy. However, it still showed some inhibition of SARS-CoV-2 induced cytotoxicity in VERO-6 cells..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM).
Targets&IC50	Tau filament:1.9μM
Cell Research	Instructions 1. Cell and tissue staining 1. Dissolve methylene blue in an appropriate solvent (such as water or buffer) to prepare a dye solution of a certain concentration. 2. Soak the cells or tissue sections in the dye solution, usually the staining time is several minutes. 3. After cleaning, observe the cell structure or distribution of target molecules under a microscope. 2. As MAO-A inhibitor and antidepressant and anti-anxiety drugs Methods: Through animal models or cell culture systems, the regulatory effect on neurotransmitters was studied. III. Research on neurodegenerative diseases Methods: Through animal models and cell models, its neuroprotective effects were studied. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Methylthionine chloride, Methylene Blue, Basic blue 9

Chemical Properties

Molecular Weight	373.9
Formula	C₁₆H₂₄ClN₃O₃S
Cas No.	7220-79-3
Smiles	O.O.O.[Cl-].CN(C)c1ccc2nc3ccc(cc3[s+]c2c1)N(C)C
Relative Density.	0.98 g/cm3 at 25℃

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 3.75 mg/mL (10.03 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6745 mL	13.3726 mL	26.7451 mL	133.7256 mL
5 mM	0.5349 mL	2.6745 mL	5.3490 mL	26.7451 mL
10 mM	0.2675 mL	1.3373 mL	2.6745 mL	13.3726 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc