ache-in-27

AChE-IN-27 is a small molecule used for high-throughput assays.

AChE-IN-60 (compound 6k) is a potent inhibitor of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with IC50 values of 27 nM and 43 nM, respectively. It also inhibits monoamine oxidase MAO-A and MAO-B, with IC50 values of 353 nM and 716 nM, respectively.

AChE/BChE-IN-27 is a brain-permeable dual inhibitor of AChE and BChE, with IC50 values of 3.72 μM and 9.65 μM, respectively, and a permeability coefficient (Pe) of 4.12. It exhibits significant antioxidant activity with an IC50 of 6.32 μM in DPPH assays and also demonstrates strong in vitro antioxidant capabilities. The compound shows metal-chelating properties and has neuroprotective potential against oxidative stress by significantly reducing intracellular reactive oxygen species (ROS). In vivo experiments reveal that AChE/BChE-IN-27 effectively restores AChE and BChE levels and improves cognitive function, indicating its potential application for Alzheimer’s disease (AD).

Graphislactone A is an antioxidant produced by Cephalosporium IFB-E001 and M. olivacea. It exhibits dose-dependent scavenging activity against DPPH radicals and metal ion-dependent hydroxyl radicals in vitro. Graphislactone A reduces peroxidation of human LDL and slows peroxidation of linoleic acid by 4.4-fold. It also inhibits acetylcholinesterase (AChE) with an IC50 value of 27 μM.