5rox

5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence in aqueous buffer with an excitation wavelength (λexit) of 580 nm (ε = 3.6×10^4/(M·cm)) and an emission wavelength (λemit) of 604 nm (ϕ = 0.94).

5-ROX, SE ([5-Carboxy-X-rhodamine, succinimidyl ester] *Single isomer*) is a labeling reagen used in the preparation of charge-modified dye-labeled Dideoxynucleotide Triphosphates (ddNTP) to "direct-load" DNA sequencing.

5-ROX tetrazine is a derivative of the ROX (Rhodamine X, Rhodamine 101) dye, characterized as a red fluorophore with high brightness and fluorescence quantum yield. This compound contains a tetrazine moiety that can undergo an inverse electron demand Diels-Alder reaction (IEDDA) with trans-cyclooctenes and other strained alkenes, exhibiting exceptional speed and specificity.

5-ROX-alkyne, a rhodamine dye, labels DNA fragments and facilitates visualization in capillary electrophoresis genotyping and gel shift experiments.