5 cfda

5-CFDA (5-Carboxyfluorescein diacetate)(5-Carboxyfluorescein diacetate) is membrane-permeant. It can be loaded into cells via incubation and hydrolyzed by intracellular esterases to 5-carboxyfluorescein that is used for labeling human intervertebral disk cells in vitro for fluorescence microscopy.

5-CFDA-AM is a cell-permeable esterase substrate used as an active probe to evaluate enzyme activity and cell membrane integrity. Electroneutral and able to enter cells at lower concentrations than CFDA, it undergoes intracellular hydrolysis by esterases to produce carboxyfluorescein, which possesses an extra negative charge, enhancing cellular retention [1].

CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

5-diacetate FAM NHS (5-CFDA SE) is a cell-permeable fluorescent tracer for use in fluorescence microscopy and flow cytometry, Ex=492 nm, Em=517 nm.