pcr

Sulforhodamine 101 (SR101) is a red fluorescent dye.

5(6)-ROX (5(6)-Carboxy-X-rhodamine) is a nucleic acid fluorescent label and rhodamine dye with Ex/Em=570/595 nm. It serves as a correction dye for real-time quantitative PCR and is widely applied in DNA sequencing and fluorescent labelling.

6-HEX is a fluorescent dye widely used in biochemistry for labeling nucleic acid sequences and real-time fluorescent quantitative PCR (qRT-PCR) assays with an excitation wavelength of 520 nm and an emission wavelength of 570 nm.

Nucleic Acid Dye Green I, a cell-impermeable fluorescent dye, is widely utilized for quantifying both dsDNA and ssDNA due to its excitation and emission peaks at 497 nm and 520 nm, respectively. Additionally, it finds applications in gene expression and RT-PCR assays.

CelGreen nucleic acid gel stain *10,000× concentrate in water* is a fluorescent dye with a unique involvement that binds to all dsDNA double helix sulcus regions.In the free state, the side strand and CelGreen emit weak fluorescence, but the fluorescence is greatly enhanced once they are combined with the double-stranded DNA. Therefore, the fluorescence signal strength of CelGreen is related to the amount of double-stranded DNA, and the amount of double-stranded DNA in the PCR system can be detected according to the fluorescence signal.

CelGreen nucleic acid gel stain *10,000× concentrate in DMSO* is a fluorescent dye with a unique involvement that binds to all dsDNA double helix sulcus regions.In the free state, the side strand and CelGreen emit weak fluorescence, but the fluorescence is greatly enhanced once they are combined with the double-stranded DNA. Therefore, the fluorescence signal strength of CelGreen is related to the amount of double-stranded DNA, and the amount of double-stranded DNA in the PCR system can be detected according to the fluorescence signal.

EDANS sodium (5-(2-Aminoethylamino)-1-naphthalenesulfonic acid sodium salt) is a donor for developing FRET-based nucleic acid probes that is widely used in real time PCR assays.

ROX Reference Dye is an inert fluorescent dye used in real-time PCR (qPCR) or RT-PCR to normalize fluorescence signals between wells. It does not participate in the PCR reaction and maintains a stable fluorescence signal throughout the process。ROX dye can correct for variations in fluorescence signals caused by uneven lighting, differences in well optics (such as droplets, bubbles), and variations in reaction mixture concentration。