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Methylene Blue

Catalog No. T6888 CAS 61-73-4

Synonyms: methylthioninium chloride, CI-52015, Tetramethylthionine chloride, Basic Blue 9

Methylene Blue (Tetramethylthionine chloride) (IC50 of 1.9 μM) inhibits tau filament formation. And it inhibits soluble guanylyl cyclase. It is also used as a dye in chromoendoscopy.

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Methylene Blue, CAS 61-73-4

Pack Size	Availability	Price/USD
50 mg	In stock	$ 30.00
100 mg	In stock	$ 42.00
200 mg	In stock	$ 54.00
500 mg	In stock	$ 76.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 97%

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Description	Methylene Blue (Tetramethylthionine chloride) (IC50 of 1.9 μM) inhibits tau filament formation. And it inhibits soluble guanylyl cyclase. It is also used as a dye in chromoendoscopy.
Targets&IC50	Tau aggregation:1.9 μM, GC:
In vitro	MB could inactivate nitric oxide directly by generating superoxide anions, it may inhibit the action of nitric oxide synthase itself. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, there are MB directly activating calcium-dependent potassium channels and enhancing the release of noradrenaline from intracellular stores.
In vivo	Methylene Blue decreases C-GMP and vascular smooth muscle relaxation by inhibiting guanylate cyclase. Pharmacokinetic studies in rodents showed that MB injects by intravascular resulting in a rapid and extensive accumulation of this drug in the central nervous system (CNS). When MB is injected intravenously, it selectively gathers in parathyroid glands, therefore is easy for the identification of structures during surgery.
Kinase Assay	Isolated Integrin Binding Assays.:Purified integrin (1 μg/mL; 4℃) is used to coat 96-well microtitre plates, which are then blocked with bovine serum albumin (BSA) (3% in 1 mM CaCl2, 1 mM MgCl2,10 pM MnCl2, 100 mM NaC1,50 mM Tris-hydroxymethyl-aminomethane; pH 7.4), and incubated (3 h at 30 ℃) with biotinylated ligands (1 pg/mL in binding buffer: 0.1% BSA, 1 mM CaCl2,1 mM MgCl2, 10 μM MnCl2, 100 mM NaCl, 50 mM Tris-hydroxymethyl-aminomethane; pH 7.4) in the presence or absence of serially diluted peptides. After washing (3×5 min with binding buffer), the bound biotinylated ligand is detected with alkaline-phosphatase conjugated goat anti-biotin antibodies (1 μg/mL; 1 h, 37℃), using p-nitrophenyl phosphate as chromogen. Cyclo (-RGDfK) binding in the absence of competitor is defined as 100% signal; binding to blocked wells in the absence of integrin is defined as 0%. Concentrations of Cyclo (-RGDfK) required for 50% inhibition of signal (IC50 values) are estimated graphically.
Cell Research	Immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are exposed at the sixth day in vitro to MB (10 μM) for 2h. Assess the survival and dendritic arbor architecture of these cells 48h after this treatment paradigm.
Animal Research	MB (5 or 50 mg/kg) diluted in normal saline were injected intraarterially into Sprague- Dawley male rats over the 30s.

Synonyms	methylthioninium chloride, CI-52015, Tetramethylthionine chloride, Basic Blue 9
Molecular Weight	319.85
Formula	C16H18ClN3S
CAS No.	61-73-4

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 3.2 mg/mL (10 mM)

DMSO: 1mg/ml, Sonication is recommended.

References and Literature

1. Vutskits L, et al. Anesthesiology. 108(4):684-692. 2. Hochgräfe K, et al. Acta Neuropathol Commun. 2015 May 10;3:25. 3. Masaki E, et al. Anesth Analg. 1999, 89(2):484-489. 4. Ginimuge PR, et al. J Anaesthesiol Clin Pharmacol. 2010, 26(4):517-520. 5. Fan Z, Tian Y, Chen Z, et al. Blocking interaction between SHP2 and PD‐1 denotes a novel opportunity for developing PD‐1 inhibitors[J]. EMBO Molecular Medicine. 2020: e11571.

Citations

1. Fan Z, Tian Y, Chen Z, et al. Blocking interaction between SHP2 and PD‐1 denotes a novel opportunity for developing PD‐1 inhibitors. EMBO Molecular Medicine. 2020: e11571

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Drug Library Inhibitor Library Microtubule-Targeted Compound Library Anti-Parasitic Compound Library Anti-Cancer Clinical Compound Library Anti-Cancer Approved Drug Library Drug Repurposing Compound Library Anti-Neurodegenerative Disease Compound Library Membrane Protein-targeted Compound Library GPCR Compound Library

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Keywords

Methylene Blue 61-73-4 Cytoskeletal Signaling GPCR/G Protein Immunology/Inflammation Microbiology/Virology Neuroscience NO Synthase Microtubule Associated Guanylate cyclase Parasite Monoamine Oxidase antimalarial anxiolytic inhibit Methylthioninium methemoglobinemias cGMP CI 52015 Nitric oxide synthases methylthioninium chloride NOS Tetramethylthionine Chloride Inhibitor antinociception CI-52015 CI52015 Tetramethylthionine chloride antidepressant dye methylthioninium Chloride MAO vasopressor Basic Blue-9 Guanylate Cyclase Basic Blue9 Basic Blue 9 inhibitor

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