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L-NAME hydrochloride

Name: L-NAME hydrochloride
Brand: TargetMol
SKU: T6570
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T6570Cas No. 51298-62-5

Alias NG-Nitroarginine methyl ester hydrochloride, N(G)-Nitro-L-arginine methyl ester, L-NAME HCl

L-NAME hydrochloride is an inhibitor of nitric oxide synthase (NOS) with an IC₅₀ of 70 μM, and it can be used to induce hypertension and kidney injury models.

L-NAME hydrochloride

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Purity: 99.93%

Catalog No. T6570Alias NG-Nitroarginine methyl ester hydrochloride, N(G)-Nitro-L-arginine methyl ester, L-NAME HClCas No. 51298-62-5

L-NAME hydrochloride is an inhibitor of nitric oxide synthase (NOS) with an IC₅₀ of 70 μM, and it can be used to induce hypertension and kidney injury models.

Pack Size	Price	USA Warehouse	Global Warehouse
1 g	$40	In Stock	In Stock
5 g	$113	-	In Stock
10 g	$163	-	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.93%

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	L-NAME hydrochloride is an inhibitor of nitric oxide synthase (NOS) with an IC₅₀ of 70 μM, and it can be used to induce hypertension and kidney injury models.
Targets&IC50	eNOS:39 nM(Ki), nNOS:15 nM(Ki)
In vivo	L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2]
Disease Modeling Protocol	Chronic kidney disease model；Hypertension model Modeling Mechanism： L-NAME hydrochloride, as a specific inhibitor of nitric oxide synthase (NOS), inhibits nitric oxide (NO) synthesis by blocking the activity of endothelial, inducible, and neuronal NOS. NO deficiency leads to vascular smooth muscle relaxation dysfunction and sympathetic nerve activation, resulting in vasoconstriction and increased peripheral resistance, ultimately leading to persistent hypertension. It is also accompanied by renal oxidative stress (glutathione metabolism disorder), renal tubular damage, and glomerular sclerosis. Related Products： L-NAME hydrochloride (T6570) Modeling Method： Experimental Subject： Rats, Sprague-Dawley (SD), Male, Body weight 330–390 g Dosage and Administration Route： ① Loading dose: 1.5 mg/100 g body weight L-NAME hydrochloride, Intravenous injection (i.v.), Single administration; ② Maintenance dose: 150 mg/L L-NAME hydrochloride solution, free access, replacing conventional drinking water; Control treatment: Control group administered equal volume saline solution via tail vein injection+conventional drinking water; Dosing Frequency and Duration Model： ① Loading dose: Single administration; ② Maintenance dose: Continuous free access for 21 consecutive days; Validation： 1. Blood pressure indicators: Systolic blood pressure, diastolic blood pressure, and mean arterial pressure were significantly elevated (p<0.05), while heart rate was significantly decreased. Blood pressure steadily increased from day 7 until the experimental endpoint. 2. Oxidative stress: Serum and renal tissue levels of reduced glutathione (GSH) were decreased, while serum levels of oxidized glutathione (Ox-GSH) were increased (p<0.05). 3. Pathological indicators: Renal tissue showed renal tubular damage, cast formation, glomerular sclerosis, and peritubular fibrosis (significantly elevated scores, p<0.05); Immunohistochemistry showed a significant upregulation of the immunoreactivity of endothelial NOS (eNOS) and inducible NOS (iNOS) in renal tissue (p<0.05). 4. Biochemical indicators: Serum sodium (Na⁺) levels were significantly decreased (p<0.05), while serum urea and creatinine levels showed no significant abnormalities, and creatinine clearance remained stable. Precautions： On day 21, after euthanizing the animals, the blood was centrifuged to separate serum (and stored at -80°C), and the kidneys were partially fixed in 10% formalin for pathological examination. A portion was flash-frozen in liquid nitrogen for the detection of oxidative stress indicators. References：Aydoğdu N,et,al. The Effects of Irisin on Nω-Nitro-L-arginine Methyl Ester Hydrochloride-Induced Hypertension in Rats. Balkan Med J. 2019 Oct 28;36(6):337-346.
Kinase Assay	Enzyme Assay: The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated.
Cell Research	rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days. (Only for Reference)
Synonyms	NG-Nitroarginine methyl ester hydrochloride, N(G)-Nitro-L-arginine methyl ester, L-NAME HCl

Chemical Properties

Molecular Weight	269.69
Formula	C₇H₁₅N₅O₄·HCl
Cas No.	51298-62-5
Smiles	Cl.COC(=O)[C@@H](N)CCCNC(=N)N[N+]([O-])=O
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (926.99 mM), Sonication is recommended.

H2O: 27 mg/mL (100.11 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7080 mL	18.5398 mL	37.0796 mL	185.3980 mL
5 mM	0.7416 mL	3.7080 mL	7.4159 mL	37.0796 mL
10 mM	0.3708 mL	1.8540 mL	3.7080 mL	18.5398 mL
20 mM	0.1854 mL	0.9270 mL	1.8540 mL	9.2699 mL
50 mM	0.0742 mL	0.3708 mL	0.7416 mL	3.7080 mL
100 mM	0.0371 mL	0.1854 mL	0.3708 mL	1.8540 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc