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Doxycycline

Name: Doxycycline
Brand: TargetMol
SKU: T1687
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1687Cas No. 564-25-0

Alias Vibramycin, Doxytetracycline, Doxycyclinum, Doxiciclina

Doxycycline is an orally active tetracycline antibiotic with broad-spectrum inhibitory effects on matrix metalloproteinases (MMPs), possessing both antibacterial and antitumor activities, and is commonly used in inducible gene expression ON-OFF systems.

Doxycycline

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Purity: 99.88%

Catalog No. T1687Alias Vibramycin, Doxytetracycline, Doxycyclinum, DoxiciclinaCas No. 564-25-0

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$30	In Stock	In Stock
25 mg	$45	In Stock	In Stock
50 mg	$72	In Stock	In Stock
100 mg	$97	In Stock	In Stock
500 mg	$173	In Stock	In Stock
1 g	$256	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.88%

Color:White to Yellow

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Doxycycline is an orally active tetracycline antibiotic with broad-spectrum inhibitory effects on matrix metalloproteinases (MMPs), possessing both antibacterial and antitumor activities, and is commonly used in inducible gene expression ON-OFF systems.
Targets&IC50	Pancreatic Cancer cells (48 h):987.5 μM, Pancreatic Cancer cells (72 h):99.64 μM, A549 cells:1.06 μM, Pancreatic Cancer cells (96 h):50.02 μM, chlamydial isolates:0.497 μg/mL, NCI-H446 cells:1.70 μM
In vitro	METHODS: Twenty-two tumor cells were treated with Doxycycline for 48 h. Cell viability was measured by MTT. RESULTS: Lung cancer cells were more sensitive to Doxycycline than most other cell lines. NCI-H446 and A549 cells were sensitive to Doxycycline with IC50s of 1.70 μM and 1.06 μM, respectively. [1] METHODS: Human pancreatic cancer cells PANC-1 were treated with Doxycycline (10-40 μg/mL) for 8-24 h. The cell cycle was detected by Flow Cytometry. RESULTS: Doxycycline caused the cells to arrest in the G1-S phase of the cell cycle. [2]
In vivo	METHODS: To detect anti-tumor activity in vivo, Doxycycline (50 mg/kg) was administered intraperitoneally once daily for fifteen days to BALB/c mice harboring human mammary carcinoma tumor 4T1. RESULTS: Doxycycline delayed tumor growth and reduced the number of tumor-associated macrophages and blood vessels. [3] METHODS: To assay antimicrobial activity in vivo, Doxycycline (40 mg/kg) was administered intraperitoneally to BALB/c mice exposed to B. pseudomallei 1026b twice daily for two weeks. RESULTS: Doxycycline-treated mice survived 100% of the time, and no B. pseudomallei DNA was amplified from the lungs or spleens of the majority of the surviving mice. [4]
Synonyms	Vibramycin, Doxytetracycline, Doxycyclinum, Doxiciclina

Chemical Properties

Molecular Weight	444.43
Formula	C₂₂H₂₄N₂O₈
Cas No.	564-25-0
Smiles	[H][C@@]12[C@@H](C)c3cccc(O)c3C(=O)C1=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@]1([H])[C@H]2O
Relative Density.	1.3809 g/cm3 (Estimated)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 200 mg/mL (450.01 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 4 mg/mL (9 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2501 mL	11.2504 mL	22.5007 mL	112.5037 mL
5 mM	0.4500 mL	2.2501 mL	4.5001 mL	22.5007 mL
10 mM	0.2250 mL	1.1250 mL	2.2501 mL	11.2504 mL
20 mM	0.1125 mL	0.5625 mL	1.1250 mL	5.6252 mL
50 mM	0.0450 mL	0.2250 mL	0.4500 mL	2.2501 mL
100 mM	0.0225 mL	0.1125 mL	0.2250 mL	1.1250 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc