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(20S)-Protopanaxadiol

Catalog No. T2799   CAS 30636-90-9
Synonyms: 20-Epiprotopanaxadiol, 20(S)-APPD

(20S)-Protopanaxadiol (20-Epiprotopanaxadiol) (20-Epiprotopanaxadiol), an apoptosis inducer, is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin.

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(20S)-Protopanaxadiol Chemical Structure
(20S)-Protopanaxadiol, CAS 30636-90-9
Pack Size Availability Price/USD Quantity
5 mg In stock $ 45.00
10 mg In stock $ 64.00
50 mg In stock $ 243.00
100 mg In stock $ 366.00
1 mL * 10 mM (in DMSO) In stock $ 46.00
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Purity: 98.91%
Purity: 98.03%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description (20S)-Protopanaxadiol (20-Epiprotopanaxadiol) (20-Epiprotopanaxadiol), an apoptosis inducer, is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin.
In vitro Concentrations causing growth inhibition of 50% of cells (LC50) for the compounds PPD is 23 μg/ml, for Int-407 cells. The the LC50 for PPD is determined to be 24 μg/ml in Caco-2 cells. In the Int-407 cell line, PPD produces great percentage of sub-G1 (apoptotic) cells(The Int-407 cell line is a human embryonic nonmalignant cell line originating from fetal tissue of approximately 2 months gestation, whereas Caco-2 is a human colon adenocarcinoma cell line). This response is attributed to differences in individual ginsenoside glycosylation and, thus, the hydrophobic-hydrophilic balance [2]. Incubation with PPD also significantly reduces the viability of U251-MG and U87-MG cells in a dose- and time-dependent manner. The cytotoxic effect of PPD is accompanied by reduced expression of cell adhesion proteins, including N-cadherin and integrin β1, which leads to reduced phosphorylation of focal adhesion kinase. Furthermore, incubation with PPD reduces the expression of cyclin D1 and subsequently induces cell-cycle arrest at the G1 phase[3].
In vivo Treatment with PPD(20(S)-Protopanaxadiol) and PPT(20(S)-Protopanaxatriol) prior to immobilization stress increase the time spent in open arms and open arm entries in the elevated plus-maze (EPM) test. Treatment with PPD potently suppresses immobilization stress-induced serum levels of corticosterone and interleukin (IL)-6 by the enzyme-linked immunosorbent assay. PPD and PPT may exhibit the anxiolytic effect via γ-aminobutyrateA (GABAA) receptor(s) and serotonergic receptor(s), respectively, and PPD may have an anti-inflammatory effect that is more potent than that of PPT[1].
Cell Research Int-407 and Caco-2 cells are seeded at a concentration of 1×106 cells/mL in 24-well plates in separate experiments. Test ginsenosides (PPD, PPT, Rh2) are added to wells to the LC50 concentration determined previously from the MTT assays. The ginsenoside concentrations used for Int-407 cells are 23, 26, and 53 μg/mL, respectively, for PPD, PPT, and Rh2. In a similar experiment using Caco-2 cells, 24 μg/mL for both PPD and PPT and 55 μg/mL for Rh2 is used. Untreated cells represented the control. Cells are incubated at 37℃ in a 5% CO2 humidified incubator for 24, 48, and 72 h. Cell-free supernatant are obtained by centrifugation(400 g) for 10 min, and the lactate dehydrogenase (LDH) assay is conducted as previously reported.(Only for Reference)
Source
Synonyms 20-Epiprotopanaxadiol, 20(S)-APPD
Molecular Weight 460.73
Formula C30H52O3
CAS No. 30636-90-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 31 mg/mL (67.3 mM)

DMSO: 85 mg/mL (184.6 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

TargetMolReferences and Literature

1. Oh HA, et al. Biol Pharm Bull. 2015, 38(2):331-5. 2. Popovich DG, et al. Can J Physiol Pharmacol. 2004, 82(3):183-90. 3. Wanderi C, et al. Anticancer Res. 2016, 36(3):925-32.

Related compound libraries

This product is contained In the following compound libraries:
Tobacco Monomer Library Neuronal Signaling Compound Library NF-κB Signaling Compound Library Metabolism Compound Library Bioactive Compound Library Anti-Obesity Compound Library ReFRAME Related Library Autophagy Compound Library Immunology/Inflammation Compound Library Kinase Inhibitor Library

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Keywords

(20S)-Protopanaxadiol 30636-90-9 Apoptosis Immunology/Inflammation Membrane transporter/Ion channel Metabolism Neuroscience NF-Κb P-gp Reactive Oxygen Species ginseng Cluster of differentiation 243 20-Epiprotopanaxadiol (20S)Protopanaxadiol 20APPD 20(S)-APPD P-glycoprotein ABCB1 CD243 (20S) Protopanaxadiol Pgp MDR1 various tumor cells Protopanaxadiol Multidrug resistance protein 1 inhibit Inhibitor saponins Akt activity inhibitor

 

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