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Immunology/Inflammation COX Ibuprofen

Ibuprofen

Catalog No. T1394   CAS 15687-27-1
Synonyms: (±)-Ibuprofe, Motrin, Brufen, Advil

Ibuprofen is a propionic acid derivate and nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic effects. Ibuprofen inhibits the activity of cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes.

Ibuprofen, CAS 15687-27-1
Pack Size Availability Price/USD Quantity
5 mg In stock 16.00
10 mg In stock 20.00
25 mg In stock 26.00
50 mg In stock 33.00
100 mg In stock 40.00
200 mg In stock 50.00
500 mg In stock 60.00
1 g In stock 75.00
1 mL * 10 mM (in DMSO) In stock 54.00
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Biological Description
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Description Ibuprofen is a propionic acid derivate and nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic effects. Ibuprofen inhibits the activity of cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes.
Targets&IC50 COX-1 : ic50 13μM,   COX-2 : ic50 370μM,  
Kinase Assay Radiochemical enzyme assays for COX-1 and COX-2: 10 μL of purified COX-1 (0.7-0.8 μg) or COX-2 (3.0 units, 0.3μg) is activated with 50 μL of cofactor solution [l-epinephrine (1.3 mg/mL), reduced glutathione (0.3 mg/mL), and hematin (1.3 mg/mL) in oxygen-free Tris-HCl buffer (pH 8.0)]. The enzyme solution (60 μL) is added to Ibuprofen solutions or DMSO (20 μL) after [14C]arachidonic acid is added in 0.2 mL eight-strip test tubes and preincubated 10 minutes on ice. Samples are incubated for 15 minutes at 37 °C, after which the reaction is terminated by addition of 10 μL of 2 M HCl and 5 μL of carrier solution (PGE2 and PGF2α, 0.2 μg/mL of each in EtOH). The unmetabolized arachidonic acid is separated from the prostaglandin products by column chromatography and eluted with n-hexane-dioxane-glacial acetic acid (70:30:1). The prostaglandin products are then eluted with EtOAc-MeOH (85:15), and the samples are counted in a Packard scintillation spectrometer. IC50 values are obtained by linear regression analysis.
Cell Research
Each cell line is incubated with Ibuprofen of various concentrations for 48 hours. Cell survival is estimated by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Cells are lysed and analyzed by western blotting for detection of the p75NTR protein.(Only for Reference)
Cell lines: Bladder epithelial cell line T24, RT-4 transitional cell papilloma bladder cell line, 5637 primary carcinoma bladder cell line, HCT-116, MDAMB231, MCF7, HEK293, A549, SKOV3 and DU145
Animal Research
Animal Model: FeSprague-Dawley rats with joint inflammation induced by high-repetition and high-force (HRHF) tasks
Synonyms (±)-Ibuprofe , Motrin , Brufen , Advil
Purity 99.70%
Molecular Weight 206.28
Formula C13H18O2
CAS No. 15687-27-1

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 39 mg/mL (189.1 mM)

Ethanol: 39 mg/mL (189.1 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

1% DMSO+30% polyethylene glycol+1% Tween 80: 20 mg/mL

Citations

References and Literature
1. Noreen Y, et al. J Nat Prod, 1998, 61(1), 2-7. 2. Palayoor ST, et al. Oncogene, 1999, 18(51), 7389-7394. 3. Khwaja F, et al. Cancer Res, 2004, 64(17), 6207-6213. 4. Rao GH, et al. Arteriosclerosis, 1983, 3(4), 383-388. 5. Driban JB, et al. J Biomed Biotechnol, 2011, 2011, 691412-691428.

Related compound libraries

This product is contained In the following compound libraries:
Approved Drug Library Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Apoptosis Compound Library Autophagy Compound Library Neuronal Signaling Compound Library Ion Channel Inhibitor Library FDA-approved Drug Library Immunology/Inflammation Compound Library Bioactive Lipid Compound Library Cardiotoxicity Compound Library Anti-Metabolism Disease Compound Library Mitochondrial Targeting Compound Library Angiogenesis related Compound Library CNS-Penetrant Compound Library

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