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Ibuprofen

Catalog No. T1394 CAS 15687-27-1

Synonyms: (±)-Ibuprofe, Advil, Motrin, Brufen

Ibuprofen is a propionic acid derivate and nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic effects. Ibuprofen inhibits the activity of cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes.

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Ibuprofen, CAS 15687-27-1

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Ibuprofen is a propionic acid derivate and nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic effects. Ibuprofen inhibits the activity of cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes.
Targets&IC50	COX-2:370 μM, COX-1:13 μM
Kinase Assay	Radiochemical enzyme assays for COX-1 and COX-2: 10 μL of purified COX-1 (0.7-0.8 μg) or COX-2 (3.0 units, 0.3μg) is activated with 50 μL of cofactor solution [l-epinephrine (1.3 mg/mL), reduced glutathione (0.3 mg/mL), and hematin (1.3 mg/mL) in oxygen-free Tris-HCl buffer (pH 8.0)]. The enzyme solution (60 μL) is added to Ibuprofen solutions or DMSO (20 μL) after [14C]arachidonic acid is added in 0.2 mL eight-strip test tubes and preincubated 10 minutes on ice. Samples are incubated for 15 minutes at 37 °C, after which the reaction is terminated by addition of 10 μL of 2 M HCl and 5 μL of carrier solution (PGE2 and PGF2α, 0.2 μg/mL of each in EtOH). The unmetabolized arachidonic acid is separated from the prostaglandin products by column chromatography and eluted with n-hexane-dioxane-glacial acetic acid (70:30:1). The prostaglandin products are then eluted with EtOAc-MeOH (85:15), and the samples are counted in a Packard scintillation spectrometer. IC50 values are obtained by linear regression analysis.
Cell Research	Each cell line is incubated with Ibuprofen of various concentrations for 48 hours. Cell survival is estimated by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Cells are lysed and analyzed by western blotting for detection of the p75NTR protein.(Only for Reference)

Synonyms	(±)-Ibuprofe, Advil, Motrin, Brufen
Molecular Weight	206.28
Formula	C13H18O2
CAS No.	15687-27-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 39 mg/mL (189.1 mM)

Ethanol: 39 mg/mL (189.1 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Noreen Y, et al. J Nat Prod, 1998, 61(1), 2-7. 2. Palayoor ST, et al. Oncogene, 1999, 18(51), 7389-7394. 3. Khwaja F, et al. Cancer Res, 2004, 64(17), 6207-6213. 4. Rao GH, et al. Arteriosclerosis, 1983, 3(4), 383-388. 5. Driban JB, et al. J Biomed Biotechnol, 2011, 2011, 691412-691428.

Citations

1. Bi X, Yan X, Jiang B, et al. Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses. Toxicology and Applied Pharmacology. 2021: 115778.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Ibuprofen 15687-27-1 Immunology/Inflammation Neuroscience COX AGS gastric cancer inhibit photosensitivity (±)-Ibuprofe Cyclooxygenase Advil angiogenesis microtubule breast cancer Apoptosis (±)-Ibuprofen T cells Neuroprotection Motrin anti-tumor inflammation anti-cancer Brufen cystic fibrosis tumor microenvironment Inhibitor inhibitor

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