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Ibuprofen

Catalog No. T1394 CAS 15687-27-1

Synonyms: Brufen, (±)-Ibuprofe, Motrin, Advil

Ibuprofen (Advil) is a propionic acid derivate and nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic effects. Ibuprofen inhibits the activity of cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes.

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Ibuprofen, CAS 15687-27-1

Pack Size	Availability	Price/USD
500 mg	In stock	$ 45.00
1 g	In stock	$ 53.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.7%

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Description	Ibuprofen (Advil) is a propionic acid derivate and nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic effects. Ibuprofen inhibits the activity of cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes.
Targets&IC50	COX-2:370 μM, COX-1:13 μM
In vitro	In the adult female Sprague-Dawley rat model induced by the HRHF method, Ibuprofen treats arthritis and early cartilage degeneration by inhibiting serum C1 and 2C as well as collagen degradation and synthesis.
In vivo	Within cells, Ibuprofen inhibits the cyclooxygenases COX-1 and COX-2, and the conversion of arachidonic acid into prostaglandin H2 (PGH2). In prostate tumor cells, Ibuprofen can suppress the activation of NF-κB and IKKα.
Kinase Assay	Radiochemical enzyme assays for COX-1 and COX-2: 10 μL of purified COX-1 (0.7-0.8 μg) or COX-2 (3.0 units, 0.3μg) is activated with 50 μL of cofactor solution [l-epinephrine (1.3 mg/mL), reduced glutathione (0.3 mg/mL), and hematin (1.3 mg/mL) in oxygen-free Tris-HCl buffer (pH 8.0)]. The enzyme solution (60 μL) is added to Ibuprofen solutions or DMSO (20 μL) after [14C]arachidonic acid is added in 0.2 mL eight-strip test tubes and preincubated 10 minutes on ice. Samples are incubated for 15 minutes at 37 °C, after which the reaction is terminated by addition of 10 μL of 2 M HCl and 5 μL of carrier solution (PGE2 and PGF2α, 0.2 μg/mL of each in EtOH). The unmetabolized arachidonic acid is separated from the prostaglandin products by column chromatography and eluted with n-hexane-dioxane-glacial acetic acid (70:30:1). The prostaglandin products are then eluted with EtOAc-MeOH (85:15), and the samples are counted in a Packard scintillation spectrometer. IC50 values are obtained by linear regression analysis.
Cell Research	Each cell line is incubated with Ibuprofen of various concentrations for 48 hours. Cell survival is estimated by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Cells are lysed and analyzed by western blotting for detection of the p75NTR protein.(Only for Reference)

Synonyms	Brufen, (±)-Ibuprofe, Motrin, Advil
Molecular Weight	206.28
Formula	C13H18O2
CAS No.	15687-27-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 39 mg/mL (189.1 mM)

Ethanol: 39 mg/mL (189.1 mM)

References and Literature

1. Noreen Y, et al. J Nat Prod, 1998, 61(1), 2-7. 2. Palayoor ST, et al. Oncogene, 1999, 18(51), 7389-7394. 3. Khwaja F, et al. Cancer Res, 2004, 64(17), 6207-6213. 4. Rao GH, et al. Arteriosclerosis, 1983, 3(4), 383-388. 5. Driban JB, et al. J Biomed Biotechnol, 2011, 2011, 691412-691428.

Citations

1. Bi X, Yan X, Jiang B, et al. Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses. Toxicology and Applied Pharmacology. 2021: 115778. 2. Jiang F, Chen Y, Yu J, et al.Repurposed Fenoprofen Targeting SaeR Attenuates Staphylococcus aureus Virulence in Implant-Associated Infections.ACS Central Science.2023

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Drug Library Inhibitor Library Anti-Cancer Approved Drug Library EMA Approved Drug Library Anti-Cancer Clinical Compound Library Drug Repurposing Compound Library ReFRAME Related Library NO PAINS Compound Library Anti-Aging Compound Library Autophagy Compound Library

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Keywords

Ibuprofen 15687-27-1 Immunology/Inflammation Neuroscience COX AGS gastric cancer Brufen inhibit photosensitivity Cyclooxygenase angiogenesis microtubule breast cancer Apoptosis (±)-Ibuprofe (±)-Ibuprofen T cells Neuroprotection anti-tumor Motrin inflammation anti-cancer cystic fibrosis tumor microenvironment Ibuprofe Advil Inhibitor inhibitor

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