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Anti-COX-2 Antibody (1O260)

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Catalog No. TMAB-00471

Anti-COX-2 Antibody (1O260) is a Rabbit antibody targeting COX-2. Anti-COX-2 Antibody (1O260) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-COX-2 Antibody (1O260)

Anti-COX-2 Antibody (1O260)

😃Good
Catalog No. TMAB-00471
Anti-COX-2 Antibody (1O260) is a Rabbit antibody targeting COX-2. Anti-COX-2 Antibody (1O260) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$272 7-10 days
100 μL$488 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Human,Mouse
Ig Type
IgG
Clone
1O260
Reactivity
Human,Mouse
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
13. 25 μg total protein per Lane of various lysates probed with Cyclooxygenase 2 monoclonal antibody, unconjugated (TMAB-00471) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
14. RAW264.7 (M) cells were treated with or without LPS+BFA (100 ng/mL) for 6 h, 25 μg total protein per Lane of cell lysates probed with Cyclooxygenase 2 monoclonal antibody, unconjugated (TMAB-00471) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMicrosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Post-translational modifications : S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundProstaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Antigen Details
Immunogen
A synthesized peptide: human COX2
Antigen Species
Human
Gene ID
5743
Uniprot ID
Biology Area
Hormone biosynthesis,Tumor biomarkers,Lipoprotein metabolism,Platelets,Cholesterol Metabolism,Macrophage / Inflamm.,Hormone biosynthesis,Cholesterol Metabolism,Lipoprotein metabolism,Heart disease,Prostaglandins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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