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Foscarnet sodium

Catalog No. T0220 CAS 63585-09-1

Synonyms: Phosphonoformate

Foscarnet sodium is an antiviral agent used in the treatment of cytomegalovirus retinitis. Foscarnet also shows activity against human herpesviruses and HIV.

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Foscarnet sodium, CAS 63585-09-1

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Foscarnet sodium is an antiviral agent used in the treatment of cytomegalovirus retinitis. Foscarnet also shows activity against human herpesviruses and HIV.
In vitro	Foscarnet Sodium is able to penetrate into cells and inhibit viral replication by inhibiting viral DNA synthesis. Foscarnet Sodium (300 μM) displays a more than 95% inhibition on HTLV-III reverse transcriptase activity in H9 cells infected with HTLV-III. [1] Foscarnet Sodium has a low cellular toxicity, but shows an inhibition of cell proliferation at high concentrations, which is reversed after drug removal. Incubation 0.5-1 mM Foscarnet Sodium with HeLa cells or primary human lung fibroblast for 24-48 hr inhibited 50% DNA synthesis and/or cell proliferation. [1] Foscarnet Sodium is able to bind with the heparin-binding domain of FGF2 and inhibits FGF2 induced cell proliferation. Foscarnet Sodium (25 μM) treating HUVEC cells for 48 hr reduced FGF2-induced cellular proliferation by 20%. Foscarnet Sodium (0.5-1 mM) reduced the 24h proliferation rate of CAL-62 cell, a Human thyroid anaplastic carcinoma cell line which expresses FGF2, to nearly 80%. [2]
In vivo	Foscarnet Sodium display an efficient therapy effect on animal with virus diseases, such as cutaneous infection, herpes keratitis, genital infection, or systemic and interacerebral infection. For example, Foscarnet Sodium (100 mM) applied at initiated 48 h post inoculation with twice daily applications of 30 μL for 4 days, displays a more than 70% reduction in cumulative score after inoculation with HSV-1 strain C42. Foscarnet Sodium (20 mg/kg) given as an aerosol to mice infected with murine CMV caused a reduction of 60% in lung titers. [1]
Kinase Assay	Reverse transcriptase assays: Reverse transcriptase assays are carried out in a reaction mixture (50 μL) containing 50 mM Tris-HCl (pH 7.5), 5 mM DTT, 100 mM potassium chloride, 0.01% Triton X-100 or NP40, 10 μg/ml (dT)15.(A)n as template primer and [3H] deoxythymidine triphospahate. The reaction mixture is incubated for 1 hr at 37 ℃ and stopped by the addition of 50 μg of yeast tRNA and 2 mL of 10% solution of trichloroacetic acid containing 1 mM sodium pyrophosphate. The samples are filtered on filters (0.45 μm), washed first with 5% TCA solution for 5 times and then with 2 ml of 70% ethanol. The filters are dried, scintillation fluid is added and the radioactivity counted in a counter.
Cell Research	CAL-62 cells are placed on 96 well plates at 8×103 cells/well in 100 μL DMEM for 24 h. Then medium is replaced by fresh DMEM with or without foscarnet. After 48 h of incubation, 20 μL of CellTiter 96AQ solution is added to the cells and incubated for 2-4 h. The quantity of formazan product as measured by the absorbance at 492 nm.(Only for Reference)

Synonyms	Phosphonoformate
Molecular Weight	191.95
Formula	CNa3O5P
CAS No.	63585-09-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 3 mg/mL (15.63 mM)

DMSO: 7 mg/mL (36.47 mM)

Ethanol: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Oberg B. Pharmacol Ther, 1989, 40(2), 213-285. 2. Rose K. Biomed Pharmacother, 2013, 67(1), 53-57. 3. Sarin PS, et al. Biochem Pharmacol, 1985, 34(22), 4075-4079. 4. Alenius S, et al. Antimicrob Agents Chemother, 1978, 14(3), 408-413.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Keywords

Foscarnet sodium 63585-09-1 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology DNA/RNA Synthesis Reverse Transcriptase Virus Protease Phosphonoformic acid trisodium replication Inhibitor inhibit retinitis Trisodium phosphonoformate Foscarnet suppression cytomegalovirus reversible antiherpesvirus DNA viral polymerase Phosphonoformate inhibitor

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