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Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865)

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Catalog No. TMAB-01446

Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865) is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, T221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865)

Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865)

😃Good
Catalog No. TMAB-01446
Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865) is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, T221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
25 μL$154 7-10 days
50 μL$271 7-10 days
100 μL$487 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

Reactivity: Human,Mouse,Rat
Ig Type
IgG
Clone
2H865
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:20-50; IF: 1:100-500; FCM: 1:20-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research Backgroundphosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
Antigen Details
Immunogen
A synthesized peptide: human JNK1 around the phosphorylation site of T183
Antigen Species
Human
Gene ID
5599
Uniprot ID
Biology Area
MAPK pathway,TLR Signaling,MAPK Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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