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Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865) is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, T221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Antibody (2H865) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 μL | $154 | 7-10 days | |
50 μL | $271 | 7-10 days | |
100 μL | $487 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM Reactivity: Human,Mouse,Rat |
Ig Type | IgG |
Clone | 2H865 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3 (T183+T183+T221) Monoclonal Antibody, Unconjugated (TMAB-01446) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:20-50; IF: 1:100-500; FCM: 1:20-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Nucleus. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53. |
Immunogen | A synthesized peptide: human JNK1 around the phosphorylation site of T183 |
Antigen Species | Human |
Gene ID | 5599 |
Uniprot ID | |
Biology Area | MAPK pathway,TLR Signaling,MAPK Pathway |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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