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Anti-PI3Kβ Polyclonal Antibody is a Rabbit antibody targeting PI3Kβ. Anti-PI3Kβ Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days |
Description | Anti-PI3Kβ Polyclonal Antibody is a Rabbit antibody targeting PI3Kβ. Anti-PI3Kβ Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (PI3-kinase p110 subunit beta) polyclonal Antibody, unconjugated (TMAB-01535) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. 2. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-1:200; IF: 1:50-200 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]. |
Immunogen | KLH conjugated synthetic peptide: human PI3 Kinase p110 beta |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Kinases/Phosphatases,TLR Signaling,Other Kinases,Lipid Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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