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Anti-PI3Kβ Polyclonal Antibody

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Catalog No. TMAB-01535
Alias PtdIns-3-kinase subunit p110-beta, PtdIns-3-kinase subunit beta, PtdIns 3 kinase p110, PK3CB, Pik3cb, PIK3C1, PI3-kinase subunit beta, PI3-kinase p110 subunit beta, PI3KCB, PI3Kbeta, PI3K beta, PI3K, PI3 kinase p110 subunit beta, PI3 Kinase p110 beta, phosphoinositide 3 kinase catalytic beta polypeptide, Phosphatidylinositol-4, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform, Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta, Phosphatidylinositol 3 kinase catalytic beta polypeptide, p110beta, p110 BETA, 5-bisphosphate 3-kinase catalytic subunit beta isoform, 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta

Anti-PI3Kβ Polyclonal Antibody is a Rabbit antibody targeting PI3Kβ. Anti-PI3Kβ Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

Anti-PI3Kβ Polyclonal Antibody

Anti-PI3Kβ Polyclonal Antibody

😃Good
Catalog No. TMAB-01535Alias PtdIns-3-kinase subunit p110-beta, PtdIns-3-kinase subunit beta, PtdIns 3 kinase p110, PK3CB, Pik3cb, PIK3C1, PI3-kinase subunit beta, PI3-kinase p110 subunit beta, PI3KCB, PI3Kbeta, PI3K beta, PI3K, PI3 kinase p110 subunit beta, PI3 Kinase p110 beta, phosphoinositide 3 kinase catalytic beta polypeptide, Phosphatidylinositol-4, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform, Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta, Phosphatidylinositol 3 kinase catalytic beta polypeptide, p110beta, p110 BETA, 5-bisphosphate 3-kinase catalytic subunit beta isoform, 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
Anti-PI3Kβ Polyclonal Antibody is a Rabbit antibody targeting PI3Kβ. Anti-PI3Kβ Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
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Product Introduction

Bioactivity
Description
Anti-PI3Kβ Polyclonal Antibody is a Rabbit antibody targeting PI3Kβ. Anti-PI3Kβ Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
SynonymsPtdIns-3-kinase subunit p110-beta, PtdIns-3-kinase subunit beta, PtdIns 3 kinase p110, PK3CB, Pik3cb, PIK3C1, PI3-kinase subunit beta, PI3-kinase p110 subunit beta, PI3KCB, PI3Kbeta, PI3K beta, PI3K, PI3 kinase p110 subunit beta, PI3 Kinase p110 beta, phosphoinositide 3 kinase catalytic beta polypeptide, Phosphatidylinositol-4, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform, Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta, Phosphatidylinositol 3 kinase catalytic beta polypeptide, p110beta, p110 BETA, 5-bisphosphate 3-kinase catalytic subunit beta isoform, 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (PI3-kinase p110 subunit beta) polyclonal Antibody, unconjugated (TMAB-01535) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
2. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
Recommended Dose
WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-1:200; IF: 1:50-200
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export.
Tissue SpecificityExpressed ubiquitously.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThis gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PI3 Kinase p110 beta
Antigen Species
Human
Gene Name
PIK3CB
Gene ID
Protein Name
Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit beta isoform
Uniprot ID
Biology Area
Kinases/Phosphatases,TLR Signaling,Other Kinases,Lipid Kinases
Function
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.
Chemical Properties
Molecular WeightTheoretical: 123 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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