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Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody

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Catalog No. TMAB-01480
Alias p-PIK3CA (Y317), p-PIK3CA (Tyr317), PIK3CA (p-Y317), PIK3CA (p-Tyr317)

Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIK3CA (Tyr317). Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody

Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody

😃Good
Catalog No. TMAB-01480Alias p-PIK3CA (Y317), p-PIK3CA (Tyr317), PIK3CA (p-Y317), PIK3CA (p-Tyr317)
Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIK3CA (Tyr317). Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIK3CA (Tyr317). Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Aliasp-PIK3CA (Y317), p-PIK3CA (Tyr317), PIK3CA (p-Y317), PIK3CA (p-Tyr317)
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-PI3KCA (Tyr317)) Polyclonal Antibody, Unconjugated (TMAB-01480) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 μg
Lane 3: Jurkat (Human) Cell Lysate at 30 μg
Lane 4: MCF-7 (Human) Cell Lysate at 30 μg
Lane 5: A431 (Human) Cell Lysate at 30 μg
Lane 6: Siha (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-PI3KCA (Tyr317) (TMAB-01480) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/120 kDa
Observed band size: 110 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundPI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human PI3KCA around the phosphorylation site of Tyr317
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Signal transducers,Signal transduction,TLR Signaling,Lipid Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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