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Anti-PIK3CA Polyclonal Antibody
😃Good
Catalog No. TMAB-01538
Anti-PIK3CA Polyclonal Antibody is a Rabbit antibody targeting PIK3CA. Anti-PIK3CA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-PIK3CA Polyclonal Antibody
Anti-PIK3CA Polyclonal Antibody
😃Good
Catalog No. TMAB-01538
Anti-PIK3CA Polyclonal Antibody is a Rabbit antibody targeting PIK3CA. Anti-PIK3CA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PIK3CA Polyclonal Antibody is a Rabbit antibody targeting PIK3CA. Anti-PIK3CA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Cow,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PIK3CA) Polyclonal Antibody, Unconjugated (TMAB-01538) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: SW480 (Human) Cell Lysate at 30 μg NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-PIK3CA (TMAB-01538) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PIK3CA) Polyclonal Antibody, Unconjugated (TMAB-01538) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PIK3CA) polyclonal Antibody, Unconjugated (TMAB-01538) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach. |
| Immunogen | KLH conjugated synthetic peptide: human PI3KCA |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Signal transducers,Signal transduction,TLR Signaling,Lipid Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Serine/threonine protein kinase PIK3CAPtdIns-3-kinase subunit αPtdIns-3-kinase subunit alphaPtdIns-3-kinase subunit aPIK3CAPI3K-αPI3KαPI3-kinase subunit αPI3-kinase subunit alphaPI3-kinase subunit aPI3K-alphaPI3KalphaPI3K-aPI3KaPhosphoinositide-3-kinase catalytic α polypeptidePhosphoinositide-3-kinase catalytic alpha polypeptidePhosphoinositide-3-kinase catalytic a polypeptidePhosphoinositide 3-kinase αPhosphoinositide 3-kinase alphaPhosphoinositide 3-kinase aPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit α isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit a isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit α (PtdIns-3-kinase subunit p110-α;p110α)Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha;p110alpha)Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit a (PtdIns-3-kinase subunit p110-a;p110a)p110αp110alphap110ap110 αp110 alphap110 a5-bisphosphate 3-kinase catalytic subunit α isoform5-bisphosphate 3-kinase catalytic subunit alpha isoform5-bisphosphate 3-kinase catalytic subunit a isoform
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