Shopping Cart

Your shopping cart is currently empty

Hemin

Name: Hemin
Brand: TargetMol
SKU: T5515
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T5515Cas No. 16009-13-5

Alias Hemin chloride

Hemin (Hemin chloride) is a chlorinated iron-containing porphyrin, a heme oxygenase (HO)-1 inducer. Hemin has therapeutic activity in porphyrias by reducing heme deficiency in patients, thereby inhibiting δ-aminolevulinic acid synthetase activity through biochemical feedback.

Hemin

Copy Product Info

😃Good

Purity: 99.59%

Catalog No. T5515Alias Hemin chlorideCas No. 16009-13-5

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$30	In Stock	In Stock
1 g	$40	In Stock	In Stock
5 g	$50	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.59%

Appearance:Solid

Resource Download

COA LCMS

Product Introduction

Bioactivity

Description	Hemin (Hemin chloride) is a chlorinated iron-containing porphyrin, a heme oxygenase (HO)-1 inducer. Hemin has therapeutic activity in porphyrias by reducing heme deficiency in patients, thereby inhibiting δ-aminolevulinic acid synthetase activity through biochemical feedback.
Targets&IC50	human caRD cellsiac voltage-gated sodium channel hNa V 1.5:80 nM, CYP3A4:2.4 μM, SARS-CoV-2:0.6805 μM (EC50), CYP1A2:27 μM, CYP2D6:19 μM, ANO1:0.45 μM
In vitro	METHODS: Pancreatic cancer cells PA-TU-8902, BxPC-3 and MiaPaCa-2 were treated with Hemin (30 µM) for 48 h. Cell viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay. RESULTS: Hemin had a significant effect on cell proliferation of PA-TU-8902, BxPC-3 and MiaPaCa-2 cell lines, decreasing cell proliferation to 62±5%, 51±3% and 38±8%, respectively. [1] METHODS: Astrocyte cultures were treated with Hemin (25 µM) for 12-24 h. Iron content was measured using colorimetric method. RESULTS: Iron accumulation occurred in cultured astrocytes after incubation with Hemin. [2]
In vivo	METHODS: To investigate the effects on renal injury, Hemin (100 µmol/kg) was administered intraperitoneally to BABL/c mice with renal ischemia-reperfusion. RESULTS: Hemin pretreatment promoted ERK1/2 phosphorylation and enhanced tubular recovery, thereby preventing further kidney injury. [3] METHODS: To investigate the effects on insulin resistance, Hemin (50 µmol/kg) was administered intraperitoneally to C57BL/6 mice on a high-fat diet once daily for four weeks. RESULTS: Hemin prevented the development of high-fat diet-induced insulin resistance by increasing insulin sensitivity in skeletal muscle. [4]
Cell Research	In vitro effects of various statins and hemin, a heme oxygenase inducer, on cell proliferation were evaluated in PA-TU-8902, MiaPaCa-2 and BxPC-3 human pancreatic cancer cell lines. The effect of statins on heme oxygenase activity was assessed and heme oxygenase-silenced cells were used for pancreatic cancer cell proliferation studies. Cell death rate and reactive oxygen species production were measured in PA-TU-8902 cells, followed by evaluation of the effect of cerivastatin on GFP-K-Ras trafficking and expression of markers of invasiveness, osteopontin (SPP1) and SOX2[1].
Synonyms	Hemin chloride

Chemical Properties

Molecular Weight	651.94
Formula	C₃₄H₃₂ClFeN₄O₄
Cas No.	16009-13-5
Smiles	[Cl-].[Fe+3].Cc1c(CCC(O)=O)c2cc3[n-]c(cc4nc(cc5[n-]c(cc1n2)c(C=C)c5C)c(C=C)c4C)c(C)c3CCC(O)=O
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (46.02 mM), Sonication is recommended.

0.1M NaOH: 25 mg/mL (38.35 mM), Sonication is recommended.

In Vivo Formulation

0.05% DMSO+99.95% 0.1 M NaoH: 0.33 mg/mL (0.51 mM), Solution.

8% 0.1M NaOH+92% PBS: 2 mg/mL (3.07 mM), Solution, pH=7.

0.05%DMSO+99.95%PBS: 0.33 mg/mL (0.51 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

0.1M NaOH/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.5339 mL	7.6694 mL	15.3388 mL	76.6942 mL
5 mM	0.3068 mL	1.5339 mL	3.0678 mL	15.3388 mL
10 mM	0.1534 mL	0.7669 mL	1.5339 mL	7.6694 mL
20 mM	0.0767 mL	0.3835 mL	0.7669 mL	3.8347 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc