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Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody
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Catalog No. TMAB-01444
Alias SAPK1c, SAPK1, PRKM8, p-JNK1 (Thr183), p-JNK1 (T183), mitogen-activated protein kinase 8, JNK-46, JNK21B1/2, JNK1A2, JNK1 (p-Thr183), JNK1 (p-T183), JNK1, JNK
Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1 (Thr183). Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody
Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody
😃Good
Catalog No. TMAB-01444Alias SAPK1c, SAPK1, PRKM8, p-JNK1 (Thr183), p-JNK1 (T183), mitogen-activated protein kinase 8, JNK-46, JNK21B1/2, JNK1A2, JNK1 (p-Thr183), JNK1 (p-T183), JNK1, JNK
Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1 (Thr183). Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1 (Thr183). Anti-Phospho-JNK1 (Thr183) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | SAPK1c, SAPK1, PRKM8, p-JNK1 (Thr183), p-JNK1 (T183), mitogen-activated protein kinase 8, JNK-46, JNK21B1/2, JNK1A2, JNK1 (p-Thr183), JNK1 (p-T183), JNK1, JNK |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Verified Activity | 1. Sample: Hela-Uv (Human) Cell Lysate at 30 μg 293T-Uv (Human) Cell Lysate at 30 μg Jurkat-Uv (Human) Cell Lysate at 30 μg Primary: Anti-phospho-JNK1 (Thr183) (TMAB-01444) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa 2. Sample: U251 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-JNK1 (Thr183) (TMAB-01444) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-JNK1 (Thr183)) Polyclonal Antibody, Unconjugated (TMAB-01444) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human JNK1 around the phosphorylation site of Thr183 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | MAPK pathway,TLR Signaling,MAPK Pathway |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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