Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody
😃Good
Catalog No. TMAB-01445
Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, T221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody
Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody
😃Good
Catalog No. TMAB-01445
Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, T221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MAPK8) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Kidney (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Cerebrum (Rat) Lysate at 40 μg Lane 4: Large intestine (Mouse) Lysate at 40 μg Lane 5: Uterus (Mouse) Lysate at 40 μg Lane 6: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 7: A431 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221) (TMAB-01445) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46/54 kDa Observed band size: 46 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221 |
| Antigen Species | Human |
| Gene ID | 5599 |
| Uniprot ID | |
| Biology Area | MAPK pathway,TLR Signaling,MAPK Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody | purchase Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody | Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody cost | order Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.