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Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody
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Catalog No. TMAB-01445
Alias Tyrosine protein kinase JAK1, Stress activated protein kinase JNK1, SAPK1, SAPK gamma, SAPK 1, Protein kinase, mitogen-activated, 8, Protein kinase JNK1, PRKM8, PRKM 8, p-JNK1/2/3 (Thr 183, 183, 221), p-JNK1/2/3 (T 183, 183, 221), p54 gamma, MK10, MK09, MK08, Mitogen activated protein kinase 8, MAPK8, MAPK 8, MAP kinase 8, JNK21B1/2, JNK1A2, JNK1/2/3 (p-Thr 183, 183, 221), JNK1/2/3 (p-T 183, 183, 221), JNK 46, JNK 1, JNK, JAK1A, JAK 1A, EC 2.7.11.24, C-JUN kinase 1, c Jun N terminal kinase 1, AI849689
Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, 221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody
Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody
😃Good
Catalog No. TMAB-01445Alias Tyrosine protein kinase JAK1, Stress activated protein kinase JNK1, SAPK1, SAPK gamma, SAPK 1, Protein kinase, mitogen-activated, 8, Protein kinase JNK1, PRKM8, PRKM 8, p-JNK1/2/3 (Thr 183, 183, 221), p-JNK1/2/3 (T 183, 183, 221), p54 gamma, MK10, MK09, MK08, Mitogen activated protein kinase 8, MAPK8, MAPK 8, MAP kinase 8, JNK21B1/2, JNK1A2, JNK1/2/3 (p-Thr 183, 183, 221), JNK1/2/3 (p-T 183, 183, 221), JNK 46, JNK 1, JNK, JAK1A, JAK 1A, EC 2.7.11.24, C-JUN kinase 1, c Jun N terminal kinase 1, AI849689
Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, 221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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| Description | Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, 221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Tyrosine protein kinase JAK1, Stress activated protein kinase JNK1, SAPK1, SAPK gamma, SAPK 1, Protein kinase, mitogen-activated, 8, Protein kinase JNK1, PRKM8, PRKM 8, p-JNK1/2/3 (Thr 183, 183, 221), p-JNK1/2/3 (T 183, 183, 221), p54 gamma, MK10, MK09, MK08, Mitogen activated protein kinase 8, MAPK8, MAPK 8, MAP kinase 8, JNK21B1/2, JNK1A2, JNK1/2/3 (p-Thr 183, 183, 221), JNK1/2/3 (p-T 183, 183, 221), JNK 46, JNK 1, JNK, JAK1A, JAK 1A, EC 2.7.11.24, C-JUN kinase 1, c Jun N terminal kinase 1, AI849689 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MAPK8) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Kidney (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Cerebrum (Rat) Lysate at 40 μg Lane 4: Large intestine (Mouse) Lysate at 40 μg Lane 5: Uterus (Mouse) Lysate at 40 μg Lane 6: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 7: A431 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221) (TMAB-01445) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46/54 kDa Observed band size: 46 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | MAPK pathway,TLR Signaling,MAPK Pathway |
| Molecular Weight | Theoretical: 42 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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