Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sivelestat (ONO5046) is a potent and selective inhibitor of neutrophil elastase with IC50 of 44 nM.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 42.00 | |
10 mg | In stock | $ 61.00 | |
25 mg | In stock | $ 110.00 | |
50 mg | In stock | $ 210.00 | |
100 mg | In stock | $ 315.00 | |
200 mg | In stock | $ 523.00 | |
500 mg | In stock | $ 837.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 68.00 |
Description | Sivelestat (ONO5046) is a potent and selective inhibitor of neutrophil elastase with IC50 of 44 nM. |
Targets&IC50 | neutrophil elastase:44 nM. |
In vitro | Sivelestat suppresses iNOS gene expression in proinflammatory cytokine-stimulated hepatocytes. Sivelestat reduces the levels of inflammatory mediators by inhibiting NF-kB. It suppresses the growth of gastric cancer cells by inhibiting the release of TGF-α stimulated by NE. |
In vivo | Administration of sivelestat attenuates pulmonary fibrosis after acute lung injury in mice. Sivelestat could attenuate sepsis-related kidney injury in rats. It blocks GAPDH/Siah1 which is a novel signaling pathway during the progression of spinal cord injury. |
Cell Research | TMK-1 Gastric carcinoma cells are seeded into 96-well culture plates (5 ×103 cells/well) in RPMI containing 7% FBS, 2.0 mM L-glutamine, and 1% nonessential amino acids. Following 24-h incubation in RPMI-1640 medium with 5% FBS, the cells are incubated for 24, 48 or 72 h at 37°C in a humidified atmosphere of 5% CO2 with varied concentrations (0.1–1.0 μg/mL) of NE, or with varied concentrations (0.1–1000 μg/mL) of sivelestat or with concentrations (10 and 100 ng/mL) of anti-TGF-α antibody in the presence of 1 μg/mL NE. After incubation, 10 μL of MTT solution (5 mg/mL) is added to each well and the plates are then incubated for 3 h at 37°C. The growth medium is then replaced with 150 μL of dimethyl sulfoxide (Wako) per well and the absorbance at 540 nm is measured. |
Animal Research | Animal Models: Male Sprague-Dawley rats. Formulation: Saline. Dosages: 50 or 100 mg/kg. Administration: i.p. |
Synonyms | ONO5046, EI546, LY544349 |
Molecular Weight | 434.46 |
Formula | C20H22N2O7S |
CAS No. | 127373-66-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 80 mg/mL (184.1 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 80 mg/mL (184.1 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Sivelestat 127373-66-4 Microbiology/Virology Others Proteases/Proteasome SARS-CoV Serine Protease ONO5046 Inhibitor ischemia LY-544349 inhibit LY 544349 Acute EI546 reperfusion EI-546 EI 546 SARS coronavirus injury ONO 5046 Elastase lung ONO-5046 LY544349 inhibitor