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MG-132

Name: MG-132
Brand: TargetMol
SKU: T2154
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2154Cas No. 133407-82-6

Alias Z-LLL-al, Z-Leu-Leu-Leu-CHO, MG132

MG-132 (Z-Leu-Leu-Leu-al) is a 26S proteasome inhibitor (IC50=100 nM) that is cell-permeable and reversible. MG-132 acts as an autophagy activator and also induces apoptosis.

MG-132

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Purity: 99.99%

Catalog No. T2154Alias Z-LLL-al, Z-Leu-Leu-Leu-CHO, MG132Cas No. 133407-82-6

MG-132 (Z-Leu-Leu-Leu-al) is a 26S proteasome inhibitor (IC50=100 nM) that is cell-permeable and reversible. MG-132 acts as an autophagy activator and also induces apoptosis.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$40	In Stock	In Stock
25 mg	$81	In Stock	In Stock
50 mg	$147	In Stock	In Stock
100 mg	$228	In Stock	In Stock
200 mg	$396	In Stock	In Stock
500 mg	$497	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.99%

Color:White

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COA HNMR HPLC MS

Product Introduction

Bioactivity

Description	MG-132 (Z-Leu-Leu-Leu-al) is a 26S proteasome inhibitor (IC50=100 nM) that is cell-permeable and reversible. MG-132 acts as an autophagy activator and also induces apoptosis.
Targets&IC50	Calpain:1.2 μM (cell free), COS-7 cells:< 10 μM, 20S proteasome:100 nM (cell free), HCT116 cells:0.82 μM
In vitro	METHODS: Human cervical cancer cells HeLa were treated with MG-132 (0.5-30 μM) for 24 h, and cell growth inhibition was detected by MTT. RESULTS: MG-132 dose-dependently inhibited HeLa cell growth with an IC50 of approximately 5 μM. [1] METHODS: Human mesothelioma cells NCI-H2452 were treated with MG-132 (0.25-2 μM) for 36 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: MG-132 treatment induces cleavage of caspases 3 and 7, Bid, and PARP in NCI-H2452 cells. MG-132 induces a caspase-dependent apoptosis. [2] METHODS: Human melanoma cells MeWo were treated with MG-132 (0.01-1 μM) for 24 h, and the cell cycle profiles were analyzed by Flow Cytometry. RESULTS: MG-132 induces cell cycle arrest at G2 phase in MeWo cells. [3]
In vivo	METHODS: To detect anti-tumor activity in vivo, MG-132 (1 mg/kg) was injected intravenously into C.B-17/lcr-scid/scidJcl mice harboring the human cervical cancer tumors HeLa, CaSki, or C33A twice a week for 4 weeks. RESULTS: MG-132 significantly inhibited the growth of human cervical cancer tumors, indicating antitumor activity in vivo. [4] METHODS: To investigate the effects of long-term treatment with MG-132 on cardiac hypertrophy and its associated molecular mechanisms, MG-132 (0.1 mg/kg) was injected intraperitoneally into rats with an abdominal aortic band (AAB) once daily for 8 weeks. RESULTS: MG-132 treatment significantly attenuated left ventricular myocyte area, left ventricular weight/body weight, and lung weight/body weight ratios, decreased left ventricular diastolic diameter and wall thickness, and increased the shortening fraction in AAB rats. MG-132 treatment significantly reversed the elevated levels of ERK1/2 and JNK1 phosphorylation in AAB rats. [5]
Synonyms	Z-LLL-al, Z-Leu-Leu-Leu-CHO, MG132

Chemical Properties

Molecular Weight	475.62
Formula	C₂₆H₄₁N₃O₅
Cas No.	133407-82-6
Smiles	CC(C)C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)OCc1ccccc1)C=O
Relative Density.	1.073 g/cm3

Storage & Solubility Information

Storage

store at low temperature | store at 4°C | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: Insoluble

Ethanol: 47.5 mg/mL (99.87 mM), Sonication is recommended.

DMSO: 240 mg/mL (504.6 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 9 mg/mL (18.92 mM), Suspension.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1025 mL	10.5126 mL	21.0252 mL	105.1259 mL
5 mM	0.4205 mL	2.1025 mL	4.2050 mL	21.0252 mL
10 mM	0.2103 mL	1.0513 mL	2.1025 mL	10.5126 mL
20 mM	0.1051 mL	0.5256 mL	1.0513 mL	5.2563 mL
50 mM	0.0421 mL	0.2103 mL	0.4205 mL	2.1025 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0210 mL	0.1051 mL	0.2103 mL	1.0513 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc