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Melatonin

Catalog No. T1659 CAS 73-31-4

Synonyms: Melatonine, N-Acetyl-5-methoxytryptamine

Melatonin is a biogenic amine that is found in animals and plants. In mammals, melatonin is produced by the PINEAL GLAND. Its secretion increases in darkness and decreases during exposure to light. Melatonin is implicated in the regulation of SLEEP, mood, and REPRODUCTION. Melatonin is also an effective Antioxidant.

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Melatonin, CAS 73-31-4

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vivo

Melatonin increases the levels of activated PTEN, RSK-1, mTOR and AMPKα kinases, mildly inhibits ERK-1/2 phosphorylation and Bad phosphorylation, significantly inhibits phosphorylations of S6 Ribosomal Protein, 4E-BP1, GSK-3α and GSK-3β, and slightly increases PRAS40 phosphorylation in animals[5]. Melatonin ameliorates the neurotoxiciy and astrocyte activation induced by Aβ1-42 in the cerebral cortex. Melatonin also blocks the reduction in Reelin and Dab1 expression induced by Aβ1-42[2]. Melatonin treatment and lack of NLRP3-/- share similar inhibition of NF-κB and NLRP3 signaling pathway in mice. Melatonin treatment and lack of NLRP3-/- share some patterns of clock genes expression, and improve cardiomyocytes morphology in mice[6].

Synonyms	Melatonine, N-Acetyl-5-methoxytryptamine
Molecular Weight	232.28
Formula	C13H16N2O2
CAS No.	73-31-4

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 11.6 mg/mL (50 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Reiter RJ, et al. J Biomed Sci, 2000, 7(6), 444-458. 2. Reiter RJ, et al. Prog Neurobiol, 1998, 56(3), 359-384. 3. Reiter RJ, et al. Cell Biochem Biophys, 2001, 34(2), 237-256. 4. Vanecek J, et al. Physiol Rev, 1998, 78(3), 687-721. 5. Kilic U, et al. Particular phosphorylation of PI3K/Akt on Thr308 via PDK-1 and PTEN mediates melatonin's neuroprotective activity after focal cerebral ischemia in mice. Redox Biol. 2017 Apr 5;12:657-665. 6. Hu C, et al. Neuroprotective effect of melatonin on soluble Aβ1-42-induced cortical neurodegeneration via Reelin-Dab1 signaling pathway. Neurol Res. 2017 Apr 7:1-1 7. Rahim I, et al. Melatonin administration to wild-type mice and non-treated NLRP3 mutant mice share similar inhibition of the inflammatory response during sepsis. J Pineal Res. 2017 Mar 31 8. Bu LJ, et al. Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation. World J Gastroenterol. 2017 Feb 14;23(6):986-998.

Citations

1. Chen J, Zhang L G, Du H X, et al. Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1‐dependent inhibition of the NLRP3 inflammasome in an EAP mouse model. The Prostate. 2021

Dose Conversion

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Melatonin 73-31-4 Apoptosis Autophagy Endocrinology/Hormones GPCR/G Protein Metabolism Neuroscience Glutathione Peroxidase Endogenous Metabolite ROR Mitophagy CaMK Melatonin Receptor Estrogen/progestogen Receptor Melatonine inhibit N-Acetyl-5-methoxytryptamine Mitochondrial Autophagy Inhibitor inhibitor

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