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E3330

Catalog No. T6823 CAS 136164-66-4

E3330 is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.

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E3330, CAS 136164-66-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 46.00
2 mg	In stock	$ 66.00
5 mg	In stock	$ 109.00
10 mg	In stock	$ 176.00
25 mg	In stock	$ 319.00
50 mg	In stock	$ 526.00
100 mg	In stock	$ 763.00
200 mg	In stock	$ 1,070.00
500 mg	In stock	$ 1,590.00
1 mL * 10 mM (in DMSO)	In stock	$ 90.00

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Purity: 98.83%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	E3330 is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.
In vitro	E3330 affects hemangioblast development in vitro via inhibition of Ape1 redox activity. [1] E3330 inhibits the growth of human pancreatic cancer cell line PANC1, XPA1, MIAPACA, BxPC3, and PK9. E3330 also promotes exit of cell cycle in PANC1 cells, inhibits the DNA-Binding activity of HIF-1α and migration of pancreatic cancer cells. [2] In JHH6 cells, E3330 prevents the functional activation of NF-κB via the alteration of APE1 subcellular trafficking and reduces IL-6 and IL-8 expression induced by TNF-α and FAs accumulation through blockage of the redox-mediated activation of NF-κB. [3]
In vivo	In mice with endotoxin-mediated hepatitis, E3330 (300 mg/kg, p.o.) attenuates the elevation of plasma tumor necrosis factor activity and protectes mice from liver injury. [4] In Rat model, E3330 (100 mg/kg, p.o.) also protectes rats from severe liver injury induced with endotoxin plus galactosamine. [5]
Cell Research	PANC1 cells are placed in one well of a 12-well plate and treated with 5 to 30 μM E3330. After a 24, 48, and 72 h of culture, the cells are washed with PBS and stained with trypan blue, and cell viability is examined by counting the live cell numbers.(Only for Reference)

Molecular Weight	378.46
Formula	C21H30O6
CAS No.	136164-66-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 70 mg/mL (185 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 70 mg/mL (185 mM)

References and Literature

1. Zou GM, et al. Blood. 2007, 109(5), 1917-1922. 2. Zou GM, et al. Mol Cancer Ther. 2008, 7(7), 22012-2021. 3. Cesaratto L, et al. PLoS One. 2013, 8(8), e70909. 4. Nagakawa J, et al. J Pharmacol Exp Ther. 1992, 262(1), 145-150. 5. Nagakawa J, et al. J Pharmacol Exp Ther. 1993, 264(1), 496-500. 6. Guerreiro PS, et al. The APE1 redox inhibitor E3330 reduces collective cell migration of human breast cancer cells and decreases chemoinvasion and colony formation when combined with docetaxel. Chem Biol Drug Des. 2017 Oct;90(4):561-571.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Active Compound Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Clinical Compound Library Bioactive Compound Library DNA Damage & Repair Compound Library Epigenetics Compound Library Orally Active Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

E3330 136164-66-4 Cell Cycle/Checkpoint Chromatin/Epigenetic DNA Damage/DNA Repair Metabolism DNA/RNA Synthesis HIF/HIF Prolyl-Hydroxylase pancreatic cancer TNF-α REF-1 NF-κB HIFs Inhibitor HIF-PH Nuclear factor-kappaB APX-3330 APE1 APX 3330 NSCLC cells neuroprotective APX3330 E-3330 endonuclease VEGFR EPCs AP-1 HUVECs Nuclear factor-κB Reactive Oxygen Species E 3330 DNA-binding Activator Protein 1 Hypoxia-inducible factors orally PANC1 anticancer H1975 PCECs inhibit IL-8 Vascular endothelial growth factor receptor inhibitor

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