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Dacarbazine

Catalog No. T1120 CAS 4342-03-4

Synonyms: DTIC-Dome, Imidazole Carboxamide

Dacarbazine is an antineoplastic agent. It has significant activity against melanomas.

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Dacarbazine, CAS 4342-03-4

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Dacarbazine is an antineoplastic agent. It has significant activity against melanomas.
In vitro	Treatment of Dacarbazine sensitizes melanomas to lysis of peptide-specific CTL and it is is mediated through Fas-independent pathway[2].
In vivo	Treatment of combination of Axitinib and DTIC demonstrates significant antitumor activity against melanoma flank xenografts, reduces tumor cell proliferation and decreases the area of tumor necrosis and increases apoptosis. It also reduces meta-tasis-related factors and prolongs lifespan in mice[3].
Cell Research	Apotosis assay: UACC903 (UACC) cells treated with or without DTIC (20 μM) for 48 h are incubated with agonist anti-Fas Ab, CH-11 (500 ng/ml), for 5 and 16 h, respectively. To distinguish whether death/apoptosis is mediated by FasR, blocking anti-Fas Ab ZB4 (2 mg/ml) is added in the corresponding groups. At the end of incubation, cells are harvested and stained with propidium iodide and FITC-annexin V (BD PharMingen) for detecting apoptosis according to the instruction provided by the company. Ten thousand cells from each group are collected, and dead and apoptotic cells are analyzed without gating using CellQuest software. (Only for Reference)

Synonyms	DTIC-Dome, Imidazole Carboxamide
Molecular Weight	182.18
Formula	C6H10N6O
CAS No.	4342-03-4

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: <1 mg/mL

DMSO: 3 mg/mL (16.46 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Serrone L, et al. J Exp Clin Cancer Res. 2000, 19(1):21-34. 2. Yang S, et al. J Immunol. 2004, 172(7):4599-608. 3. Zhang XH, et al. Oncol Lett. 2013, 6(1):69-74.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Dacarbazine 4342-03-4 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis Nucleoside Antimetabolite/Analog B cell inhibit antineoplastic Inhibitor DTIC-Dome T cell metastatic malignant melanoma Imidazole Carboxamide inhibitor

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